ISSN: 2640-7604
International Journal of Veterinary Science and Research
Research Article       Open Access      Peer-Reviewed

Species complex of mosquitos captured by mechanical traps and an equine host

Clinton B Campbell1, Daniel L Kline2*, Jerome A Hogsette2 and Saundra H TenBroeck3

1Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611, USA
2Department of Agriculture, Agricultural Research Service, Center for Medical, Agricultural, and Veterinary Entomology, Gainesville, FL 32604, USA
3Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
*Corresponding author: Daniel L Kline, Department of Agriculture, Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL 32604, USA, Tel: 352-374-5933; Fax: 352-374-5922; E-mail:
Received: 02 April, 2020 | Accepted: 11 December, 2021 | Published: 13 December, 2021
Keywords: Mosquito magnet pro trap; Mosquito magnet liberty trap; Mosquito magnetX trap; CDC-1012 trap; Culex nigripalpus

Cite this as

Campbell CB, Kline DL, Hogsette JA, TenBroeck SH (2021) Species complex of mosquitos captured by mechanical traps and an equine host. Int J Vet Sci Res 7(2): 169-177. DOI: 10.17352/ijvsr.000097


© 2021 Campbell CB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Field studies were conducted to evaluate the efficacy of commercially available mosquito traps to capture suspected West Nile Virus (WNV) vectors and compare the relative abundance, and species composition of mosquitoes captured by traps. Traps included the Mosquito Magnet (MM) Pro, MM-Liberty, MM-X, the CDC-1012, and the Home and Garden (HGM) prototype trap. In the first study, conducted at the University of Florida Horse Teaching Unit (HTU), Gainesville, FL, from May 17 to September 30, 2002, the CDC-1012 caught significantly more mosquitoes than the other traps. The HGM trap did not catch any mosquitoes and was replaced with a horse on July 17. Percentages of WNV vector species captured in traps and vacuumed from the horse, respectively, were Culex nigripalpus Theobald 85-91% and 27%, and Mansonia titillans (Walker) 2-5% and 40%. In the second study, performed for 5 days in April 2003, the MM-Pro, MM-X, and CDC-1012 traps caught significantly more mosquitoes than did the MM-Liberty trap. All traps captured primarily Cx. salinarius Coquillett, Cx. erraticus (Dyar and Knab), and Anopheles crucians Wiedemann. The horse attracted a greater percentage of Ma. titillans and less Cx. erraticus than did the mechanical traps. The mechanical traps may offer horses some degree of protection from suspected WNV species, however, a large number of Ma. titillans were recovered from the horse but the traps captured very few.


West Nile Virus (WNV) is a mosquito-borne arbovirus of the family Flaviviridae. It infects birds primarily but can also infect humans and other animals such as horses. Infection can result in death. WNV was first reported in the US in Suffolk County, NY, in 1999 [1]. It subsequently spread across the country to the Pacific coast and is a major threat to equine health. In Florida, Culex salinarius Coquillett and Cx. nigripalpus Theobald are considered to be the major vectors in the spring and in the late summer and fall, respectively [2].

WNV was first detected in Jefferson County, FL, on July 3, 2001, when a dead crow was confirmed positive for the virus [3]. Between 2001 and 2003, 1,108 (5.4%) of the clinical WNV equine cases in the US were reported from Florida [4]. Although that number has decreased in recent years, WNV continues to be a threat to Florida’s multibillion-dollar equine industry.

Most of the data linking mosquitoes to equine hosts have come from blood-meal assays and traps placed in stables [5]. Although horses appear to be a favorite host for a few species, such as Aedes vexans (Meigen) [6], many other species feed on horses occasionally. These include Anopheles crucians Wiedemann, Coquillettidia perturbans (Walker), Culiseta inornata (Williston), Cx. quinquefasciatus Say, Cx. salinarius , Cx. tarsalis Coquillett, Psorophora columbiae (Dyar and Knab), Ps. ferox (von Humboldt) [7], Cx. nigripalpus [8] and Ae. dorsalis (Meigen) [9]. Some of these species, primarily the Culex spp., have been listed as competent WNV vectors under laboratory conditions [10].

Because WNV has become a problem across the US, research in monitoring techniques is warranted. When the primary vectors have been better defined, appropriate surveillance methods can be employed by mosquito abatement districts to target these species. Mosquito surveillance traps, some of which are promoted by their manufacturers to provide some degree of protection from WNV vectors, could play a role in the collection of pertinent mosquito species. The objective of this study was to determine if an attraction differential exists between an equine host and selected commercial mosquito traps sold for surveillance and control of putative WNV vectors. These include the counterflow geometry traps sold under the Mosquito Magnet name and the ubiquitous CDC trap. The question to be answered is do the horse and the traps attract the same species complex of mosquitos.

Materials and methods

Study site

Trapping was performed at the University of Florida Horse Teaching Unit (HTU) (Figure 1), a 26-ha equine facility located 5 km south of the main campus. The HTU maintains a Quarter Horse herd of varying ages. The study site was selected because of the high mosquito pressure, the presence of numerous small ponds, and nearby wetlands with low lying areas that have a tendency to flood during rainy periods.


The CDC-1012 trap (John W. Hock Company, Gainesville, FL) attracts mosquitoes with a 6.3–Watt incandescent light plus CO2 and pulls them into a catch container with a small in-line fan (Figure 2A). The trap is powered by a rechargeable 6-v battery and CO2 is supplied by compressed gas cylinders at a rate of 500 ml/min. For our study, the CO2 line was taped to the underside of the trap lid above the fan. A polypropylene container with a 30-mesh screen bottom was used to hold the captured mosquitoes.

The Mosquito Magnet (MM) X is an American Biophysics Corporation (ABC) (North Kingston, RI) Counterflow Geometry (CFG) trap [11] (Figure 2B). The CO2 from compressed gas cylinders was supplied at a rate of 500 ml/min. The trap has a clear PVC shell, with removable top and bottom lids to allow access to the trapped insects. The trap has two fans powered by a 12-v battery.

The Home and Garden (HGM) prototype trap (BugJammer Inc., Pennington, NJ) (Figure 2C) has acoustical and visual cues for attracting mosquitoes and other insects. The unique attractant is the digitized recording of the heartbeat of a dog, which is emitted from a speaker. Electrical power is supplied by four D-cell batteries. Visual attractants include light reflected in the ultraviolet and visible ranges and contrasting black and white vertical stripes. Insects are captured on a cylindrical white adhesive sleeve placed around the housing over the speaker.

The MM-Pro (Figure 2D) is another ABC CFG trap. This trap catalytically converts propane into 3 attractants: CO2, heat, and moisture. Propane is supplied by a standard 20-lb commercial tank. The trap also generates its own power through a thermoelectric module, which allows for stand-alone operation. The MM-Pro is constructed of stainless steel with a PVC shell. Captured mosquitoes are retained in a nylon net within the trap.

The MM-Liberty (Figure 2E), another ABC CFG trap, is similar in form and function to the MM-Pro, however, the power supply is normally 120-v US alternating current. Because the traps used in this project had to be completely portable, the trap was modified by fitting its electric cord with an adaptor so the trap could be connected to a rechargeable 12-v battery. Like the MM-Pro, the MM-Liberty creates CO2 from propane combustion and mosquitoes are retained in a nylon net located inside the trap.


Two adult geldings were used to attract mosquitoes so their mosquito species profiles could be compared with those of the traps. From July 17 – August 18, 2002, a cremelo gelding was used, and from August 19-September 30, 2002, and April 2003, a chestnut gelding was used. Mosquitoes that landed on the horse were captured with a BioQuip (Rancho Dominguez, CA) portable vacuum aspirator, powered by a cigarette lighter in a vehicle. Capture canisters fit snugly inside the aspirator and the vacuum holds mosquitoes inside the canister when the power is on. A cap is placed over the canister before the vacuum is switched off.

Experimental design

At the HTU, traps were set in a 5 x 5 Latin square design, placed at 1 of 5 pre-selected sites prior to the first trapping interval then rotated sequentially through the remaining sites after each interval to constitute 1 replication. Traps were placed as follows: on opposite sides of a small pond (Figure 1A and B), near a second small pond (Figure 1C), along a pasture fence line (Figure 1D), near a large oak tree and horse barn (Figure 1E).

In the first study conducted from May 17- September 30, 2002, 5 different traps were operated then rotated through trap sites as described above. Traps were operated one night per week and then one consecutive 5-night period each month. Trapping began 2-3 h before sunset and ended 2-3 h after dawn. On July 17, 2002, the HGM trap was removed from the study and replaced with a horse. The horse was led each night to the site previously designated for the HGM trap, and mosquitoes were vacuumed from the horse for 30 min, starting approximately 15 minutes before sunset and ending 15 minutes after sunset. The remaining traps were operated through the night as previously described.

The second study, also a 5 x 5 Latin square design, was conducted on April 7, 8, 9, 15, and 16, 2003, to compare the MM-Pro, MM-Liberty, MM-X, CDC-1012, and a horse. All traps were started approximately 15 minutes before dusk and were collected 15 minutes after dusk. Meanwhile, mosquitoes were vacuumed from the horse during the same interval. Traps were emptied after the 30-minute interval, then remained operational overnight. The contents were collected the following morning. The traps and the horse were rotated after each trapping interval so that each occupied every site once during the 5-day experiment.

Captured mosquitoes were returned to the laboratory, euthanized and stored in a freezer. Identifications were made by C.B.C. using the keys of Darsie and Ward [12].

Data analysis

Numbers of mosquitoes captured were subjected to analysis by GLM after transformation by log10 (n + 1); means were originally separated using Fisher’s Least Significant Difference Test [13]. However, there are more appropriate tests for this purpose and significance levels resulting from the Fisher’s test have been disregarded. Unfortunately, raw data have been lost over the years and an updated analysis cannot be performed. Therefore, only numeric inferences will be made by means. To indicate the degree of diversity in the mosquito populations, a Shannon-Weaver index [14] was calculated using the following function:

H’ = -∑ pi ln pi

where pi = the proportion of the number of individuals of species i in the total sample number (ni/N), and ln pi = log10 pi.


Study 1

The total number of mosquitoes collected from May 17-September 30, 2002, was 272,034. During these studies, the CDC-1012 trap captured a mean number of 2,875 mosquitoe,s which was approximately 2X greater than those captured by any of the three CFG traps; the CFG trap means were clustered within a range of 245 mosquitoes, with the MM-liberty trap having the highest value and the MM-Pro trap the lowest (Table 1). The BugJammer trap did not have a CO2 source and did not catch any mosquitoes during the study.

From May 17 - July 16, 2002, 9 species of mosquitoes were captured in the largest numbers, with no single species dominating the percentages captured (Table 2). However, the three traps that captured the highest percentages (≥ 20%) of Cx. salinarius were the CDC-1012, the MM-Pro, and the MM-X. The species captured in the highest percentage (30%) by the MM-Liberty was Cx. nigripalpus, but the second highest was Cq. perturbans. Although the species captured in the highest percentage by the CDC-1012 was Cx. salinarius , surprisingly, the second highest was Ma. titillans (Walker) (Table 2). The largest total number of mosquitoes was captured by the MM-Pro and the smallest by the MM-Liberty. The total number of mosquitoes captured during this period by all four traps was 1,046.

During the remainder of study 1, from July 17 - September 30, 2002, Cx. nigripalpus was captured in the highest percentages by the traps; however, Ma. titillans , Cx. nigripalpus, and Ae. infirmatus (Dyar and Knab) were the species captured in the highest percentages from the horse (Table 3). Mansonia. titillans and Ae. infirmatus were also captured in the mechanical traps, but they did not constitute more than 4.7 and 1.6%, respectively, of any trap catch. A lower percentage of Cx. nigripalpus, but higher percentages of Ae. infirmatus , and Psorophora spp. were vacuumed from the horse than were captured in any of the traps. A total of 18 different species were trapped during this study, including Ae. albopictus (Skuse), Ae. vexans, An. crucians, An. quadrimaculatus Say, Cx. erraticus (Dyar and Knab), Cx. nigripalpus, Cx. salinarius , Cx. quinquefasciatus , Cq. perturbans, Ma. titillans , Ae. infirmatus , Ae. mitchellae (Dyar), Ps. ciliata (Fabricius), Ps. columbiae, Ps. cyanescens (Coquillett), Ps. howardii Coquillett, and Uranotaenia lowii Theobald, however, only the species constituting ≥0.03% of the total catch are shown (Table 3).

The relative abundance of 4 major species groups trapped from May 17-Sept 30, 2002, at each trapping site was very similar. Between 85 and 92% of all mosquitoes captured at all sites was Cx. nigripalpus (Table 4). Mansonia titillans, Ae. infirmatus , Psorophora spp. and other various species constituted between 7.3 and 0.69% of the catches at all sites.

Species diversity from May 17-July 16, 2002, as indicated by the Shannon-Weaver Diversity Index, was lowest for the MM-Liberty (0.84) and ranged between 0.87 and 0.93 among the remaining traps (Table 5). When the horse was included in the trapping design, from July 17-Sept 30, 2002, the species diversity for the horse was higher (0.69) than those of all three traps (0.23-0.30).

Study 2

In the 30-min trapping studies, the mean trap counts ranged from 21.8-91.6 mosquitoes, with a mean of 28.4 mosquitoes collected from the horse. The highest mean numbers of mosquitoes were captured by the MM-Pro, CDC-1012, and MM-X traps, and the lowest by the MM-Liberty trap (Table 6). The mosquito means for the horse (28.4) and the MM-Liberty trap (21.8) were relatively close, but the difference between the mosquito means for the MM-Pro, CDC-1012, and MM-X traps and the mosquito mean for the horse were ≥ 2X (Table 6). The total mosquitoes captured from the 4 traps and the horse for 5 catch periods was 1,336.

The percentages of the various mosquitoes captured during the 30-min studies differed between the traps and the horse. Although the most abundant species for all traps was Cx. salinarius , the range of percent capture differed among traps (Table 7). The CFG traps captured 65.8 - 80.1 % Cx. salinarius , but only 40.1 and 49.2 % Cx. salinarius were vacuumed from the horse and captured by the CDC-1012, respectively. Mansonia titillans recovered from the horse and trapped by the CDC-1012 represented 29.58 and 19.70%, respectively, of the total mosquitoes captured, but just 41 - 9.17% M. titillans were captured by the CFG traps. The horse attracted a higher percentage of Ae. vexans than did the traps, but the traps attracted a higher percentage of Cx. erraticus than did the horse. An. crucians abundance varied among all traps, ranging from 4.37 to 16.80 % (Table 7).

The overnight mean catches showed a distinct division between the MM-Liberty and the other traps with the MM-Liberty catching ≥ 3X fewer mosquitoes (mean = 114.6 per night) than any other trap (Table 8). The mean catches of traps other than the MM-Liberty ranged between 342.4 and 493.4 mosquitoes per night. The total catch for all traps during the 5-night trapping period was 6,693.

The relative abundance of mosquito species captured differed somewhat between the MM-Pro and the other traps in the 2003 overnight trial. Between 54 and 68% of all mosquito species captured in traps other than the MM-Pro were Cx. salinarius (Table 8). However, the MM-Pro had 84.58% Cx. salinarius , and no other species it captured represented >7 % of the total count. The other traps caught a greater percentage of Cx. erraticus and An. crucians than did the MM-Pro. Ma. titillans was ≤ 3 % of the mosquito species in any trap (Table 8).

The April trapping study yielded 16 species of mosquitoes. These included Ae. albopictus, Ae. vexans, An. crucians, An. quadrimaculatus, Cq. perturbans, Cx. erraticus, Cx. nigripalpus, Cx. salinarius , Ma. titillans , Ps. ciliata, Ps. columbiae, Ps. ferox, Ae. infirmatus , Ae. mitchellae, Ur. lowii, and Ur. sapphrina (Osten Sacken) (Table 8).

Relative abundance of mosquito species captured was similar for each site. Cx. salinarius was the primary species captured at each site, ranging between 60.7 and 75.2 % of the species composition (Table 9). Other species of a higher percentage capture were Cx. erraticus and An. crucians.

Species diversity, as indicated by the Shannon-Weaver Diversity Index, was lowest for the MM-Pro traps during the 30-minute (0.37) and overnight (0.28) trapping studies. In the 30-minute trapping studies, the species diversity was higher for mosquitoes vacuumed from the horse (0.69) than for those captured in the traps (0.37-0.63) (Table 5).


Species composition and diversity

North Florida has a unique mosquito species composition where Cx. salinarius occurs in high numbers during the winter and spring, and Cx. nigripalpus occurs primarily in the summer and fall. It is speculated that WNV-positive Cx. salinarius are overwintering the virus, and when spring temperatures are particularly warm, a large outbreak of WNV could occur in north Florida because of the early occurrence of Cx. nigripalpus [2]. This is one reason why monitoring devices, such as the traps used in this study, are important for early detection of a possible viral outbreak. Data from both studies performed at the HTU showed that Cx. salinarius was captured in spring (Table 8) and Cx. nigripalpus was the primary mosquito captured in the summer and fall months (Table 3). This is in agreement with data from other areas in Florida [2,15] and previous research performed at or near the HTU [16,17].

The relative composition of mosquito species feeding upon the horses differed from that of any of the mechanical traps, despite the horse being rotated through the same trapping sites as the traps. The composition of species in the spring would indicate that Cx. salinarius was the vector of interest most readily trapped in mechanical traps at the HTU (Table 7). Cx. salinarius is known to feed readily on both avian and mammalian hosts [18], thus the possibility of finding it both in the traps and on the horse seems quite probable during periods of peak emergence. A higher percentage of Cx. salinarius was found in the mechanical traps (49.24-80.13%) than on the horse (40.14%), but Cx. salinarius was the primary species captured from the horse as well as in the traps. An. crucians, another species known to have a broad range of hosts, was found in varying percentages in all traps. Cx. erraticus, an ornithophilic species [19], was found in higher percentages in the traps than on the horse. Thus, the data acquired during the spring would point more towards mechanical traps catching primarily species of mosquitoes that have a broad pattern of both avian and mammalian hosts.

In contrast, the horse attracted a greater variety and higher abundance of species, most known to feed on mammalian hosts, than did the mechanical traps. While Cx. salinarius was the most abundant species (40.14%) found on the horse, Cx. salinarius was found in a greater abundance in the mechanical traps (49.24-80.13%). Ma. titillans represented 29.58% of the total catch from the horse, while the CDC-1012 trap captured 19.7% and the CFG traps captured between 4.13 and 9.17 % during the 30-minute spring studies (Table 7). Ae. vexans, another species known to feed primarily on mammals [18,20], was found in greater percentages on the horse (7.04%) than in the traps (0-2.4%).

During the spring, the relative abundance of species captured during the 30-minute study was found to be similar to the overnight trial with a few exceptions. The three main species captured by all traps and on the horse were Cx. salinarius , Cx. erraticus, and An. crucians. The primary species captured in both trials was Cx. salinarius , and the difference in the proportion of Cx. salinarius captured in the 30-minute study compared to the overnight study did not differ by more than 5% for any trap (Tables 7,8). The primary exception is in the percentage of Cx. erraticus captured. Cx. erraticus populations are lower at dusk than during the remainder of the evening, as reflected by the 30-minute study at sunset and then the overnight study (Tables 7,8). This agrees with Snow [21] who recorded I activity throughout the night. The percent of Ma. titillans captured was also lower in the overnight collection when compared to the 30-minute period, especially with the CDC-1012 and the MM-Liberty traps. The percentage of An. crucians captured in the overnight trials increased slightly from the 30-minute trials in all traps except the MM-X.

Differences in percentages of mosquito species captured during the summer and fall were even more dramatic than in the spring. Between May 17 and July 16, Cx. salinarius populations were still large enough to compete with Cx. nigripalpus populations in percentages captured, at least in the MM-Pro and MM-X traps. The CDC-1012 captured a larger percentage of Cx. salinarius and the MM-Liberty captured a higher percentage of Cx. nigripalpus (Table 2). Between July 17 and September 30, between 85 and 91% of the total catch by all mechanical traps (Table 3) was Cx. nigripalpus, a species known to shift host preference from birds to mammals [8]. Other species, some of which are mammalian feeders, captured during this experiment included Ma. titillans , Cx. erraticus, and Ae. infirmatus , but none represented > 5% of the total catch of any trap in overnight trials. All mechanical traps showed their ability to attract and capture large numbers of Cx. nigripalpus, considered to be an important WNV vector in north Florida, but they did not trap any other species in large numbers during this study.

The horse had a very different species composition than the traps during the summer and fall studies. Similar to the spring studies, mosquito species found on the horse were typically mammalian feeders or had an affinity for both avian and mammalian hosts [18]. Ma. titillans was the species found in the greatest abundance on the horse, representing 40% of the total catch. This contrasts dramatically with the mechcanical traps, none of which captured Ma. titillans in percentages > 4.73. (Table 3). Cx. nigripalpus represented only 27% of the species found on the horse, which contrasted with the higher percentages (85.45 - 90.98 %) captured by the mechanical traps during this same period. Other mammalian feeders found in higher abundance on the horse than in the traps included Ae. infirmatus , and Psorophora spp. Ps. cyanescens, a species rarely found locally in mosquito traps, represented 6.65% of the mosquitoes vacuumed from the horse.

The Shannon-Weaver Diversity Indices indicate a relative degree of diversity for the mosquito species captured in traps or vacuumed from the horse (Table 5). Lower values indicate less diversity and more competition between the mosquito species for the resource being offered, i.e. the traps and the horse. Higher values indicate more diversity and less competition between the species [14]. Indices for the four traps during the May 17-July 16, 2003, study ranged from 0.83-0.94, indicating a relatively high and similar degree of diversity (Table 5). No trap captured > 31% of a single species and the other species captured constituted < 25% of the catch (Table 2). During the July 17-Sept 30, 2002, study the horse had a relatively high degree of species diversity while the traps had a very low degree of species diversity (Table 5). For the horse, three mosquito species constituted between 15.63 and 39.76 % of the catch. For the traps, Cx. nigripalpus represented between 85.73 and 91.13 % of the catch, with all other species representing ≤ 4.73% of the catch. During the 30-minute trapping study in April 2003, the diversity index of the horse was again greater than those of the traps for the same reason given above (Table 5). During the overnight study in April 2003, indices for the traps were lower than those in the May 17-July 16, 2002, study because of a higher percentage of captures of a single species, Cx. salinarius .

All mechanical traps evaluated, except the HGM without CO2, can capture large percentages of suspected WNV vector species during the spring, summer, and early fall months. If mechanical traps are shown to be an effective means of preventing vector species from feeding on host animals, i.e., horses in this case, these would be useful tools for horse owners. The mechanical traps have the ability to attract Culex spp. in a given area and, on average, capture hundreds to thousands of these mosquitoes in a single night (Tables 1,8). However, the question remains as to whether these traps give a true reflection of an actual mosquito population. The species diversity of mosquitoes vacuumed from a primary host that differs from the species diversity of mosquitoes captured in mechanical traps in the same area indicates an attraction differential between the host and the traps. If traps were used for reduction of WNV vectors such as Cx. nigripalpus on equine facilities, perhaps the virus could be somewhat controlled in a given area; however, the primary species feeding on the horses, such as Ma. titillans , would be reduced by a lesser extent.

Trap catch

In previous studies, CFG traps have been shown to have a higher mosquito yield than non-CFG traps [22,23]. However, our data indicate that the CDC-1012 trap performed as well or better than CFG traps in spring (Tables 6,8) and in summer and early fall (Table 1) under our study conditions of these studies. In all our studies, the primary mosquitoes captured by traps were Culex spp., Cx. nigripalpus in particular. Cx. nigripalpus is known to be attracted to light [24] and the light in the CDC-1012 trap in our study may be why it performed as well as or better than the CFG traps.

This study shows that CDC-1012 and CFG traps have the ability to capture host-seeking mosquitoes despite the presence of large mammalian hosts in the immediate vicinity. Although the CDC-1012 trap did have a higher average rate of catch rate during the 2002 study than the other traps, the CFG traps were able to catch large numbers of mosquitoes each night as well.

Among the CFG traps, the MM-Liberty captured the fewest mosquitoes in the 2003 study. The MM-Pro and MM-X had similar mean catch rates throughout both studies, despite a different source of CO2 for each trap.

The mosquito attractant, 1-octen-3-ol, was not used in these studies because some WNV vectors, such as Cx. nigripalpus, have a low affinity for this attractant [25-27]; however, Cx. salinarius is the exception [26,28]. Data from the 2002 study (Tables 2,3) might suggest that a large mosquito population consisting primarily of Cx. nigripalpus exists at the HTU. However, many species of Aedes, Anopheles, Coquillettidia, Mansonia, Psorophora, and Aedes are captured in larger numbers when CO2 is coupled with octenol [25,26,28]. The addition of 1-octen-3-ol may have changed the relative abundance of mosquito species captured in the mechanical traps by reducing the capture of some of the species originally targeted. This demonstrates some of the problems associated with capturing multiple species of targeted mosquitos with a single combination of traps and attractants.

Trapping site differences

The HTU is a large facility, and trap sites were located in unique areas. Some trap sites were located within a few feet of a water source, while other sites were located in open pasture. Relative abundance of individual species of mosquitoes trapped did not differ much by site in any study. During the 2002 study, Cx. nigripalpus was the primary mosquito trapped at every site, and all sites showed relatively similar results for every other species trapped (Table 4). Results were similar for the 2003 study, but Cx. salinarius was the primary mosquito trapped at each site and all species were similarly abundant at all sites (Table 9). Based on trap catches, the mosquito population appeared to be generally uniform throughout the trapping area, and large numbers of Culex mosquitoes were highly attracted to all but the HGM traps at all trap sites.


The mechanical traps captured different species profiles than the horse and generally captured more total mosquitoes than the horse during the same trapping interval (Table 7). If the species profile of the mosquitoes recovered from the horse is the one the traps should also be attracting and capturing, improvement is needed to enable traps to do so at a rate equal to or better than that of the horse. The inability of traps to outcompete the host have been documented with other dipteran species [29]. It is interesting to note that mosquito species attracted by and those not attracted by 1-octen-3-ol were all vacuumed from the horse during the same trapping intervals. This reveals some of the prevalent limitations in current technology that prevent traps from competing more effectively with animal hosts.

We thank Michael Behrens for his assistance with the high-resolution photos.

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