Hygienic practice, microbial quality and physco-chemical properties of milk collected from farmers and market chains in Eastern Wollega zone of Sibu Sire districts, Ethiopia

Thirty samples of fresh cow milk was taken from farmers immediately after milking and 15 milk samples was taken from market chains and analyzed for quality. From the sample about 31.08% were coagulate on clot on boiling test and 55.55% samples were coagulated on alcohol tests. Overall mean total bacterial counts, coliform and yeast and mould count were 5.74±0.10.65, 3.14±0.72 and 3.71±0.83 cfu/ml respectively and signifi cantly different b/n producers source and markets channel source at (P<0.05). The highest total colony count was 6.77±1.1cfu/ml was observed at retailers. From the samples about 66.7% of the sample was in a normal range for specifi c gravity and 33.3% of the sample was not in a range of normal specifi c gravity. The overall mean of fat, protein, solid not fat were 4.65±0.50%, 3.67±0.05%, 8.78±0.15% respectively. In general the result indicated that milk samples collected from producers and market chains, were subjected to microbial contamination and does not meet the international milk quality standard. Therefore, adequate sanitary measures should be taken at all stages from production to consumer level. Research Article Hygienic practice, microbial quality and physco-chemical properties of milk collected from farmers and market chains in Eastern Wollega zone of Sibu Sire districts, Ethiopia Chala Adugna1* and Mitiku Eshetu2 1Department of Veterinary Science, College of Agriculture and Veterinary Science, Ambo University, P.O. Box 19, Ambo, Ethiopia 2School of Animal and range science, Haramaya University, P.O. Box 138 Dire dawa, Ethiopia Received: 02 January, 2021 Accepted: 15 March, 2021 Published: 17 March, 2021 *Corresponding author: Chala Adugna, Department of Veterinary Science, College of Agriculture and Veterinary Science, Ambo University, P.O. Box 19, Ambo, Ethiopia, E-mail:


Introduction
Milk is the most important and precious natural material which has been the basic component of human nutrition for long period of time [1]. It is also an excellent medium for growth of microbes like bacteria which spoil and deteriorate milk quality which is not safe for human consumption [2]. Milk can be spoiled due to different factors like health of animal, from milking environment, feed and mikers , diseased udder, storage temperature [3]. Milking and storage equipment commonly used by households are believed to be inconvenient for hygienic cleaning and cause quality deterioration of milk and impose health risks on the consumers [4].
Milk must be free from pathogenic organism that causes milk borne diseases. Contamination of milk can leads milk to be spoiled which not suit for human consumption.

Description of the study area
Sibu Sire is one of the 18 districts of East Wellega zone, which is located in the eastern part of the zone. Sire is the capital town of the Woreda located on the way to Nekemte at a distance The dominant livestock species in the study area were cattle, small ruminants, mule, horse and poultry ( Figure 1).

Study design and sampling method
The study involved both cross-sectional survey method to There is tertiary microbial contamination which occurs mainly due to re-contamination of milk after being processed due to unhygienic conditions and poor or improper handling and storage of milk during consumption (Parekh, 2008). The quality of milk is determined by its composition and overall hygiene. It is well known that the fresh milk contains some bacteria and somatic cells. These are the milk's biological constituents. The numbers of these biological constituents change according to production conditions like the animal's health and hygiene during milking, preserving and transporting the milk and the milk products. These microorganisms have an important role in the alteration and contamination of milk and milk products. Temperature control is essential to prevent milk alteration, because of the microbial growth. The number of microorganisms vary according to the temperature (season) indicating that the total number of coli forms and E. coli signifi cantly differ in summer and winter. There is an increasing focus on milk quality and hygiene in the dairy industry. Producing high quality milk requires effective udder health programs at a herd level. The safety of milk is an important attribute for consumers of milk and dairy products.
Milk and products derived from milk of dairy cows can harbour a variety of microorganisms and can be important sources of food borne pathogens. There is lack of information on hygienic practice, physco-chemical composition and microbiological quality of raw cow milk in the country in general and study area in particular. So, current study is conducted with the objective to assess hygienic practice, physco-chemical properties and microbiological quality of raw cow milk produced and marketed in study area. Microbial analysis: Total aerobic plate count. Appropriate decimal dilution was selected and samples were thoroughly mixed and serially diluted by adding 1mL of the test portion into 9 mL of 0.1% sterile peptone water. Dilutions were made so that plate counts range between 25 and 250 colonies were counted [5]. Appropriate dilutions were placed on Petri dishes and pour plated with 10 to 15 mL molten plate count agar (about 45°C) and allowed to solidify for 15 minutes and incubated for 48 hours at 37°C. Finally, counts were made using a colony counter. The plate counts were calculated by multiplying the count on the dish by 10n in which n stands for the number of consecutive dilutions of the original sample [6].
Coli form count: After appropriate dilution was made by transfer 1 ml of each sample or a decimal dilution on to a sterile plate, and then added to each plate of 15 to 20 ml of VRBA tempered to 44 to 46 0 C. An agar control for each fl ask of medium used was poured. The number of samples to be planted in any one series was selected so that there was no more than a 20-minute time lapse between diluting the fi rst sample and pouring the last plate in the series (Michael &Joseph, 2004).

Yeast and Mould Count (YMC):
Sterile agar medium (250 ml portions in prescription bottles or fl asks, autoclaved 15 min at 121°C) was prepared and then tempered to 45 ±1°C in water bath. Once medium has been tempered, it was held for 2-3 hr before use, provided water level of water bath was 2-3 cm above surface of agar in aliquot container. The potato dextrose agar was used as medium growth [6].
After counting and recording bacterial colonies in each Petri dish, the number of bacteria in milliliter milk was calculated by the following formula given by American Public Health Association [7]. When computing TBC, CC and YMC only the fi rst two signifi cant digits were recorded and the bacterial count was reported as colony forming unit per millitter of milk (CFU/ml).

Data management and statistical analysis
The General Linear Model (GLM) procedure of SAS version 9.1 (2002) was used to analyze milk microbial quality and properties of raw milk. Microbial count data was fi rst transformed to logarithmic values (log 10 ) before subjected to statistical analysis in order to make the frequency distribution more symmetrical. Mean comparisons was done using the Least Signifi cant Difference (LSD) technique when analysis of variance shows signifi cant differences between means. Differences was considered statistically at p<0.05 level of signifi cance.
The following model was used for the analysis of milk microbial quality of milk, physical and chemical properties of milk (Table 1).

Milking and hygienic practice
In all of the study area cows were hand milked and calves allowed to suckle dams prior to milking and suckling were used to stimulate milk letdown. In the study area milking practice was mainly carried out by woman and males were rarely involved in milking of the cows. About 37.5% of respondent in the midland wash their hands before milking while 62.2% of the respondent do not wash their hands. In the lowland parts of the study area only 5.5% of the respondent washes their hands before milking, and 94.5% of the respondents don't wash their hands before milking and this was due to lack of awareness and scarcity water. Relatively midland (37.5%) respondents wash their hand before milking than lowland (5.5%) respondents. This result was in opposite with report of (Bekele,2015) who reported that 100% of the respondents in Dangila town of western Amhara region washes their hands before milking.
In all of the study area about 22.5% washes their hand before and after milking and 77.4% of the respondents don't washes their hand before and after milking ( Table 2). This was due to scarcity of water and lack awareness and this leads to poor quality milk. This fi nding was different from the report of [8] who reported that all of the interviewed respondents wash hands and milking vessels before milking cows in Bahir dar Zuria.
Washing and cleaning the udder of the cows before milking is the most important and the crucial thing for hygienic practices of milk production. The washing of the udder removes the dirty materials from the udder of the cows. This is because the udder of the cow has direct contact with dirty materials like urine, dung, and feed refusal [9]. As observed in this study, 66.15% of the respondent washes the udder of the cow before milking and 33.85% did not wash and simply allowed their calves to suckle before milking which is considered as the calves removes the dirty of the teat and facilitate the letdown of milk. This fi gure is greater than the report of (Saba, 2015) about quality assessment of cattle milk in Adea Berga and Ejerie districts of West Shoa zone, Ethiopia.
Hygienic practice related to cleaning milk equipment and frequency of cleaning are among the major factors affecting the quality of milk and milk products. Milk get easily be contaminated by microorganism if not properly handled. Majority of the respondents interviewed uses plastic materials for milking and storing of the milk after milking except some of the respondents from lowland area (Table 3). According to [10] report similar result in Ezrha district of Gurage Zone where all of the respondents used plastic containers as milking materials. Milking and milk storage utensils should be properly cleaned and maintained if not it can spoil the milk and milk product easily since the milk is an easily perishable product. Therefore, cleaning, and draining of equipment after each milking is important for reduction of milk microbial contamination. Producers should pay particular attention for the type as well as cleanliness of milk equipment they use for milking. Most of the respondent from highland Agro-ecologies clean their equipment with cold water but in the midland and lowland of the study area some of them use hot(boiled) water for cleaning of milking materials. Hot water was better for cleaning of milking materials as it can reduce number bacterial multiplication.

Standard plate count
The analysis of the ANOVA show that there was signifi cance difference in bacterial load among highland, midland, and lowland and market chain source of milk in study area at  The number in the bracket is the percentage of respondent from the three Agroecologies.

Yeast and mold count
The overall mean value of YMC were not signifi cantly different (P<0.05) among milk samples collected from the three agro ecologies (Table 5), but the mean value of YMC count were signifi cantly different between milk source from the three Agro-ecologies and the milk source from hotels and consumers at (P<0.05). The total YMC count of samples from market hotels and consumers were higher than the mean YMC of the Agro-ecologies, this might be due to poor hygiene of equipment during handling, transporting and processing of milk, and indicates unsanitary conditions of handling and contamination from environment.
The overall mean of YMC for the study area was 3.71±0.83log10 cfu/l. The result was similar with report of [15] who reported 3.902±0.477 in Smallholders in Bench Maji-Zone, Southwestern Ethiopia and greater than the result of Haile (2012) who report that the total count of YMC of sample of milk taken from the udder was 3.03log10 cfu/ml in Hawassa, Southern Ethiopia. It also shows higher YMC than the report of (Amakelew, et al.) who reported that the YMC of raw cow milk collected from farmers, hotels, and dairy cooperatives in Dawa Chefa district, Amhara region, Ethiopia were (0.46 log10), (0.74 log10 cfu/ml) and (0.62 log10) respectively. The increased amount of yeast and mould in the study area could be due to the use of contaminated equipments, and poor hygienic P<0.05 (Table 5). From the whole raw milk sample collected, the bacterial quality of milk from retailers was poorest in bacterial quality with total plate count of 6.77log10 cfu/ml and signifi cantly different from highland samples.
The overall mean of total bacterial count of raw cow milk produced in the study area was 5.74log10 cfu/ml. It is within acceptable range of Ethiopian microbial standard for However, the value is not in the acceptable range of total bacterial count of raw milk of European standard. According to EU standards, total bacterial count of raw cow milk should be less than 5.6log10cfu/ml [12]. However, in the present study, the total bacterial count of all milk samples exceeds the mentioned standard of European Union.
In general the high bacteria count of raw cow milk in the study area could be due to the initial contamination

Coli form count
Analysis of variances indicated that there were signifi cance difference at P<0.05 among the highland, and consumer source of milk in study areas (Table 5). Difference might be attributed to factors like low hygiene during milking, contact of the udder with faecal material and poor quality of milking equipments.
The overall mean of the coliform count in the study area were 3.14log10 cfu/ml. This result is lower than the result of [13] in Bahir dar Zuria and Mecha district, Ethiopia who reported that 4.49 logcfu/ml and it is also lower than the report of [14] who reported that the total coli form counts of milk in Yabello district Borena southern Ethiopia were 6.323 log10 cfu/ml).  N is the total number of samples taken* Citation: Adugna  The overall mean of total solids content of the current study was 13.42% and it was lower than the report of Gurmessa, et al, [19] who reported 15.47% in Borena Zone, Yabello district. The result of the current study was in a normal range given for total solid by Ethiopian standard that stated the total solid of cow milk should not be less than 12.5 % (DES, 2008). The highest total solid was recorded at highland (14.72%) of the study area. There was a signifi cance difference between highland and lowland midland and hotels source of milk at (p<0.05). The variation of total solid of the cow in the study area was due to breed, lactation stage and type of feed consumed. The mean of sample collected from study area was within normal range of Europeans standard.
The SNF content of the milk was determined by subtracting fat content from total solid of the milk. The solid not fat content of the current study was signifi cantly different between highland and other source of milk ( Table 7). The highest SNF content was recorded at highland source of milk. The average SNF content of the current study was within the standard of Ethiopia for zebu cows which indicate that the SNF not be less than 8.5% (DES, 2008) and lower than the report of Bekele, et al. (2015) who report 9.49% for urban and peri-urban area of Dangila in western Ahmara Region, and [19] 9.47 ±0.17% in case of Borena zone Yabello district.

Correlation between hygienic practice and milk quality:
The analysis of data indicated that the milking hygienic condition of the milkers. The increased count of yeast and mould from production to market is due to contamination of milk with barn bedding, farm environment, poor hygiene of milkers, use of poor quality water and fecal wastes in the farm and poor quality of transporting materials.

Physical properties of milk
Specifi c gravity and pH of the Milk: The specifi c gravity value the samples collected from the study area was in a range of 1.024-1.053 (Table 6). The normal specifi c gravity of milk at 15.6 0 c ranges from 1.026 to 1.032 (DES, 2008). The result of the study indicated that 66.7% of the samples were within normal range for specifi c gravity. Samples collected from producers(Highland, midland and Lowland) were within normal range for specifi c gravity but, samples collected from market channels were not in the normal range of specifi c gravity and this indicate that milk from market channel samples, fat was extracted. The result of this was in agreement with the report of [16] who report that 85% of Ejerie and 65%  Table 7). The midland and consumer source of sample was signifi cantly different in protein content at P<0.05. The highest protein content was recorded at highland of the study area. The average protein content of the current study was higher than the report of [17] who report 3.420 ± 0.1% at dairy farm in Dire Dawa town Eastern Ethiopia.
The overall mean protein content of the current study fulfi lls the requirement given Ethiopian standard for protein content of horro breed cows. According to ES standard the protein content Zebu cow protein content should not be less than 3.2%.
There was a signifi cant difference (P<0.05) in the average fat content of cow milk between the three agroecology. The highest milk fat content was recorded at highland with 6.19 % ( Table 7). The average fat content of the current study was greater than the report of [16] 3.52% in Ejere and Adaa Berga  practice like hand washing, cleaning of milking equipment, cleaning milking area, washing of udder have direct effect on microbiological quality of milk consumed and marketed in study area. Types of transportation and cooling of milk affects the microbial quality of milk collected from market chains in the study area. This indicated that there was a positive correlation between hygienic practice and microbial quality of milk collected from farmer and market chains and the fi nding was in agreement with the report of [20] who indicated that hygienic practice have signifi cant effect on milk microbial quality in Girar Jarso district of Oromia regional state. According to [21] also reported similar result in Cheha district of Guraghe Zone,southern Ethiopia.

Constraints of hygienic milk and milk product
productions: The major constraints of clean milk and milk product production in the study area were ranked based on the frequency of respondents in the study area. The constraints were ranked for each of the Agro-ecologies and summarized in Table 8. All of the respondents reported that lack of awareness was the major constraints of milk production in the study area [22][23][24][25][26][27]. Ranking was based on the frequency of respondents*

Conclusion and recommendation
Generally, the microbiological quality of milk collected from the study area were within the acceptable range of Ethiopian unprocessed dairy product but not in acceptable range European dairy standard due low hygienic practice and use of un cleaned material for storage and transportation.
Specifi c gravity and PH of sample collected from the study area were within normal range of Ethiopian dairy standard except sample collected from market channel. The chemical composition is within the acceptable range of Ethiopian dairy standard but in a range of European. Based on this Proper sanitization, cleaning and proper transportation storage as well as at farmer level should be maintained to ensure that milk is in good quality for consumption. Therefore, good milk handling practice should practiced, training should be provided for both milk producers and stakeholders and actors involved in milk marketing and transportation in the study area.