Effect of watering frequency on germination and early growth of maize (Zea mays) seed

Maize (Zea mays) is one of the most important cereal crops grown principally during the raining season in Ethiopia which is commonly used for both human consumption and poultry feed. Green house experiment on the effect of watering frequency on germination and early growth of maize was carried out at Ambo Agricultural Research Center using a hybrid maize variety (Jibat) (Zea mays) from December 1 to January20th, 2018/19. The study was conducted to determine the effect of watering frequencies on germination and early growth of maize seed (Zea mays). The highland maize hybrid variety of AMH-851 (Jibat) was used as a test crop for the experiment. Three watering frequencies one day interval, three days intervals and fi ve days intervals were used as an experimental treatment, and they were laid out in a Completely Randomized Design (CRD) with four replications. The watering frequencies showed statistically signifi cant differences, on plant shoot length and biomass weight, but the treatments didn’t reveal statistical difference on seedlings emergence. Therefore, one day watering interval shows better performance on shoot length, fresh and dry biomass. Research Article Effect of watering frequency on germination and early growth of maize (Zea mays) seed Gudisa Hailu1*, Tsega’ab Tesfaye1 and Rosa Ayalkibet2 1Ethiopian Institute of Agricultural Research, Ambo Plant protection Research Center P.O. Box 37, Ambo, Ethiopia 2Ambo University, Ambo, P. O. Box 19, Ethiopia Received: 23 July, 2020 Accepted: 11 February, 2021 Published: 13 February, 2021 *Corresponding author: Gudisa Hailu, Ethiopian Institute of Agricultural Research, Ambo Plant protection Research Center P.O. Box 37, Ambo, Ethiopia, E-mail:


Introduction
Irrigation can be described as the artifi cial application of water to soil for the purpose of agricultural production. It is primarily considered when there are suspected problems with rained crop production. Rainfall may be unreliable in amount and duration of timing. For instance, rainfall may vary from year to year, or the onset and cessation of the rains may be uncertain (Gevens, 2007). Meanwhile, there may be period of drought during the rainy season itself. Irrigation systems are considered because they solve the problem of food production and the materials needed or used for the practice are not as scarce as they appear to be. Effective irrigation will infl uence the entire growth and yield process from seedbed preparation, germination, root growth, nutrient utilization, plant growth regrowth, yield and quality [1]. Water is essential for irrigation purposes, but its indiscriminate use can lead not only to shortages, but also to the deterioration of crop yields and soils.
It is hence vital to ensure that it is applied as effectively as possible in order to reach sustainability. Maize production was increased with a combination of deep tillage and good irrigation system. Maize (Zea mays) is one of the most important cereal crops grown principally during the raining season in Ethiopia which is commonly used for both human consumption and poultry feed. The local production of the crop is not suffi cient to meet the continuous increase of consumption. Therefore Deborah, et al. [2] compared three methods of irrigations under climatic conditions such as wind, drip and furrow irrigation, she then came up with the conclusion that drip and furrow irrigation methods are preferred to sprinkler. Humphreys [3], while comparing the same irrigation methods under slope condition submitted that sprinkler and drip irrigation are preferred to furrow irrigation. Whether sprinkler, drip or furrow, a signifi cant effect has been realized on the growth and yield of a crop when a system is properly designed [4].

Experimental location
The experiment was undertaken in Ambo Agricultural Research Center, one of the Ethiopian Institute of Agricultural Research located. The research centre is located in West Shewa Zone of Oromia Regional state. The site is located at 116 km west of Addis Ababa (The capital city of the Ethiopia) having an altitude of 2185 m.a.s.l., latitude of 8.9658333 N and longitude of 37.85556 E with mean annual minimum and maximum temperatures of about 11 0 C and 26 0 C, respectively. According to metrological data obtained from Ambo Agricultural Research Center the area receives mean annual rainfall of 1244 mm with uni-modal rainfall pattern. Similarly, according to long term metrological data from this station the mean minimum mean maximum and average air temperatures of in the study area is10.

Experimental material
Sterilized soil was fi lled in 8 cm diameter clay pots and sown with maize seed in Ambo Agricultural Research center green house. Four seeds were sown per each pot evenly and with equal sowing depth. Maize hybrid variety -Jibata was used as planting material. The pots were arranged in CRD with four replication in a greenhouse such a way that 4 pots were watered at day interval, four pots with the three days interval and four pots with the fi ve days interval were watered using an equal volume of water for consecutive 20 days. The twelve pots (15cm diameter) height of the pots are 20 cm were used to triplicate twelve treatment combinations (6×2) with a two factor factorial completely randomized design. The pots were lined with plastic bags from inside to restrict leaching. Each pot was fi lled with 10 kg soil after mixing combining. The pots possess holes at the bottom to suck water during watering.

Experimental design and data analysis
The treatment was arranged in Completely Randomized Design (CRD) with four replications. Data collected were number seeds emerged, shoot length and biomass weight. The collected data were analyzed using standard data analysis method using MSTATC (Version 9.4) programmed software and mean separation was made using Duncan's Multiple Range Test at alpha level of 0.05 (=5%). Seeds emerged Procedure was take after 20 day interval emerged Seeds was count. It was calculated by counting normal seedlings after 20 days from planting. If seed is large the emerging seedling has a larger food source to depend on before it gets established. Seedlings from bigger seeds tend to emerge more successfully and are more vigorous both at the start of their life and throughout their whole life. Shoot length (cm) after 20 days from planting the seedling biomass was gently washed to remove the soil and shoot length was measured of each pot and each treatment seedlings (cm). Biomass weight after evaluating shoot and root lengths, from each pot 4 seedlings were dried in a forced air at green house at room temperature 28°C for 5 days to obtain seedlings dry weight and expressed as grams. The number of seedlings were limited to only four, because of the experiment was designed for the green house and to use the advantage of the pot size.
Irrigation frequency is the number of days between irrigation periods without rainfall. Crop irrigation requirement is the portion of the water consumptive use of crop, which must be supplied by irrigation to ensure optimal crop growth and development. With the rising human population and resultant increase in daily demand for maize for various uses, it becomes imperative to intensify effort at all year-round Maize Production in the country. Traditional crop of maize is greatly affected by various weather conditions. The rain in the country is very uncertain and may either cause prolonged drought or water-logged conditions. It is, therefore, essential to consider both aspects of water requirement to produce 1 kg of dry matter is the lowest in case of maize. Thus, very high water use effi ciency can be achieved by maize as compared to other cereal crops. Although maize needs less water requirement for germination, it needs 10 to 30 times greater than that of wheat and mustard. According to (Rathore, 2005), it is very critical to have enough moisture for the maize seed, during germination so that successful seed germination may occur. In Ethiopia, there is no adequate information on early time water requirement of maize.

Soil type
According to FAO classifi cation, the soil type of the study area is Vertisols [5]. The soil of study area is clay dominated and the soil textural class is clay [6]. Vertisols are clay rich soils contain a type of expansive clay that shrinks and swells dramatically. Vertisols is categorized in problematic soil due to its waterlogging problem. However, Vertisols are highly fertile soil type than red soils due to their high clay content with high cation exchangeable capacity [5]. The soil type is characterized as follows Table 1.

Results and discussion
The effect of watering frequencies on germination and early growth of maize seeds were observed under different watering frequencies. As shown in Table 2 A total of 16 seeds were used for each interval (One day interval, three-day interval and Five-day interval) and two counts were done during the present studies. The fi rst count was done fi ve days after sowing. In these rounds a total of 16 seed were used for each of the three intervals. The present studies result elucidates that continuous application of enough moisture via watering in three-day interval enhance relatively higher (50%) percentage of germination followed by one day interval (43.75%) (Figure 1). This implied that moisture stress negatively affects root initiation earlier even in pot growing conditions as in fi eld conditions. This is related with a work done by Konopka, et al. [9], irrigation intervals of 14 or 21 days initiated an earlier growth of seminal roots in the seedlings than when irrigated more frequently. The delay in root emergence with frequent irrigations could be due to the diffusion of water into the seed for its physiological processes of early growth But maintaining adequate amount of moisture in the soil fasten early emergence. The second count was done seven days after sowing. In these rounds a total of 16 seed were used for each of the three intervals. The result showed that the application of water or providing enough moisture at fi ve-day interval enhance or promote relatively better germination performance (68.75%) followed one day interval (56.25%) ( Figure 2). Table 3, watering frequencies on all the treatments had no signifi cance effect on number of maize seedlings emergence.

As shown in
Watering frequencies had signifi cance effect on maize shoot length, therefore, as date of watering elongated the shoot length consistently and signifi cantly reduced (Table 4).  2  3  3  3  16  11 68.75% Table 3: Effect of watering frequency on number of maize seedlings emerged at Ambo, 2019.

Treatment Emergence seedlings
One day interval 4a Three days interval 4a

Conclusion
The research work determined the effect of watering frequency on emergence and early growth of maize seedlings.
The early growths of maize are greatly affected by the different watering frequency. So that, the different watering frequencies considerably affected plant shoot length, green biomass weight and dry biomass weight, however, these studies have revealed there is no signifi cant difference among the treatments (One day interval, three-day interval and fi ve-day interval) in terms of maize seed emergence. So that we concluded that moisture however is critical for seedling emergence in the treatments used in the study.