Contribution to the improvement of cotton seed storage at the new textile and cotton industrial company (SN-CITEC) in Bobo-Dioulasso in Burkina Faso

The oil produced by SN-Citec is a vegetable oil made from cotton seeds. The cotton seeds from the cotton plant go through several stages or workshops to give the oil. To obtain a quality oil, it is necessary to control the physico-chemical parameters such as humidity, water content, acidity level, fat content during the storage of the raw material which is cotton. The objective of our study was to contribute to the improvement of the storage of the raw material at SN-Citec: cotton seed. We determined the physico-chemical parameters of four (4) batches of seed after storage. The values obtained were compared to the standards of the codex stand. Thus standard methods were used to evaluate the quality of storage. The results of the analyses of the parameters during storage varied from 4.84% to 5.80% for the water content, 4.77% to 4.94% for the moisture content, 2.53% to 3.65% for the acidity content and 20.28% to 20.45% for the oil content of the seed. All the results found were in accordance with codex standards and the values in the laboratory’s archives. This study showed that the oil from SN-Citec is of good quality so to obtain an oil of hygienic and nutritional quality a good method of conservation of the raw material, a check of the moisture content of the seed before and during storage, a reserved environment and a good internal temperature of the materials during storage are important. Research Article Contribution to the improvement of cotton seed storage at the new textile and cotton industrial company (SN-CITEC) in Bobo-Dioulasso in Burkina Faso Kiessoun Konaté1,2*, Dominique Ouryagala Sanou1,2, Balamoussa Santara1,2 and Mamoudou Hama Dicko1 1Laboratory of Biochemistry, Biotechnology, Food Technology and Nutrition, Department of Biochemistry and Microbiology, University Joseph Ki-ZERBO, 03 BP 7021 Ouagadougou, Burkina Faso 2Faculty of Applied Sciences and Technologies, University of Dedougou, Burkina Faso Received: 30 September, 2020 Accepted: 27 October, 2020 Published: 30 October, 2020 *Corresponding author: Kiessoun Konaté, Laboratory of Biochemistry, Biotechnology, Food Technology and Nutrition, Department of Biochemistry and Microbiology, University Joseph Ki-ZERBO, 03 BP 7021 Ouagadougou, Burkina Faso, Tel: 0022670766237; E-mail:


Introduction
Cotton (Gossypium hirsutum) is an important industrial crop worldwide and the predominant natural fi ber in the textile industry. Despite competition with artifi cial fi bers, cotton remains important and accounted for 30% of the more than 82 million tons of textile fi bers processed in 2013. Burkina Faso's industry is essentially manufacturing and oriented towards the processing of local raw materials. Among local raw materials, the oilseed sector, particularly the cotton sub-sector, is the most represented. It is Burkina Faso's second largest export sector after gold. The country therefore has a very good regional position as a seed cotton producing country [1]. Cotton seed production increased from 115,000 tons in 1985 to 631,000 tons in 2005. This sector thus represents a considerable economic stake for the country. Moreover, among the challenges facing the African cotton sector in general and that of Burkina Faso in particular is the issue of local processing of the cotton fi ber and seed produced [1,2]. Thus, emphasis has been placed in particular on the agri-food sector [3]. Nowadays, several food processing Citation: Konaté  units have been born in our country, especially oil mill units. Most of these companies have made huge investments to set up a workshop for receiving and storing cotton seeds in order to modernize their production infrastructure. The SN-Citex has a cylindrical tank called silo, two sheds and a terraced air for storing cotton seed. Seed storage is the beginning of the oil mill chain and determines the quality of the oil. At the SN-Citex the cotton seeds are then stored in the silo after analysis of certain number of parameters such as moisture,acidity. Then they are dried during the month of February and March in a open air under and equipped space and fi nally they are deposited in bulk under a hangar.These operation should make it possible to preserve the original quality of the cotton. If the cotton seed received by SN-Citex is good then it is necessary to ensure that the storage conditions do not compromise the quality of the fi nished product and that the seed has kept its original quality. It is in this perspective that this work was undertaken on the theme: "Contribution to the improvement of cotton seed raw material storage at SN-Citec". Thus, the objective of our work was to determine the physical and chemical parameters around storage in order to control the factors infl uencing storage and to take corrective measures.

Study area
Our study was carried out at the new company of oil and soap (SN Citec) located in the industrial zone 19, rue 20.231 Bobo Dioulasso. It was created in 1995 and is the fi rst agroindustry in Burkina Faso.

Biological material
The cotton seeds used for this study came from the different ginning factories of the Fibers and Textile company or Gourma cotton company for them, these seeds constitute a vegetative waste.

Experimental materials
We used an oven 103°C ± 2°C, a precision analytical balance ±0 001g and a desiccator to determine the moisture, acidity and fat content of seed batches. In addition, petri dishes were used to determine in particular the moisture content of the seed. A magnetic stirrer, a hot plate, 50ml measuring cylinder, 95° alcohol, 0.1N NaOH, 250ml Erlenmeyer fl ask, phenolphthalein, 250ml beakers, magnetic bars were also used to determine the acidity level of the seed and Soxhlets,mechanical mill, fi lter paper, extraction conductor, 200 to 250ml fl asks, hexane, 250ml beakers were used to determine the fat content.

Sampling
In the course of our work we have selected four (4) seed lots. We took three samples per lot every week. The collected seeds were put in appropriate plastic bags and transported directly to the laboratory for analysis.

Determination of the moisture content of the seed
The water content was determined according to the standard method NF EN ISO 662 (2016) [4]. The seed was therefore heated in an oven at 103±2°C until the water was completely eliminated in order to determine the loss of mass. At least three measurements were made and the arithmetic mean of these different measurements was calculated.
The moisture content of the seeds was given as a percentage by the following formula:

M M M M
With: M is the weight of the petri dish in grams, M1 is the weight of the petri dish + the mass of the test sample in grams before drying, M2 is the weight of the petri dish + the mass of the test sample in grams after drying.

Determination of the acidity level
The acidity level is the measure of free fatty acids. It has been determined thanks to the international standard NF EN ISO 660 ) [5]. At least three measurements were carried out and the arithmetic mean of these different measurements was calculated.
The expression of the acidity results was:

Determination of the fat content of cottonseed
The fat content is the percentage of oil that can be extracted from this seed. It has been standardised according to the international standard NF EN ISO104 [6]. At least three measurements were made and the arithmetic mean of these different measurements was calculated.
The method of calculating the fat content of cottonseed was as follows: Hence: P1 the weight of the empty fl ask in grams, P2 the weight the fl ask containing the extracted fat in grams, PE the mass of the Test Sample.

Results and discussion
Water content of the cotton seed per month (May and June) The water content of the seed of the lots is presented in the water content of the seed. Moreover, these quantities of water could be important to guarantee the quality of the seed which will be used to obtain an oil suitable for consumption [7]. Indeed, water plays a role as an accelerating factor in enzymatic (lipases and lipoxygenases) and non-enzymatic From Figure 1 we observed that the moisture content varied from 4.77 to 4.94. There was also a high moisture content in lots 1 and 4, which also contained a fairly high amount of water compared to the other samples ( Table 1). The amount of water is therefore proportional to the moisture content. According to Cisse [9] the moisture content of the seed is directly related to the moisture content. The wetter the seeds are, the more likely it is that a high moisture content will be observed [9].
Good seed drying and storage may help to reduce this value [9]. Similarly, the results found during the months of May and  Table 2. This could be explained by the fact that the seeds were well dried and the internal temperature as well as the humidity of the storage place had been controlled [10]. The humidity level was also high during the months of December and January. This could be due to the temperature and the beings living in the storage environment. In fact they can through respiration infl uence the humidity of the environment and therefore the moisture content of the seed.

Acidity of the cotton seed
According to Table 3 the acidity values of the seed vary from 2.53 to 3.65 and are below the codex alimentarius standard (06%) and the Burkinabe standard [6,11]. These data obtained refl ect the fact that some inconveniences, the main ones being unpleasant odour and taste, and enormous losses in refi ning had been avoided. Kassamba [12] had shown that the acidity level of the cotton seed is favoured by moisture or humifi cation of the seed or by seed ageing. We could conclude that the seeds used in our study were young and were stored at a good temperature. Table 4 shows the acidity level of the seed during storage from December to June. The results for May and June were also in accordance with the NBF standard and the Codex Alimentarius [6,11]. Moreover, they are similar to those in the laboratory's archive during the reception of the cottonseed.
Given that moisture favours acidity according to Kassamba [12] this could mean that there was no major variation in seed moisture over the last few months. Table 5 indicates that the fat content results were in the range 20.28 to 24.45%. These values are considered normal the better they are lower than the codex alimentarius standard according to which the oil content of cottonseed is 20 to 24% and the Burkinabe standard [5,9]. These results of the fat content could be explained by mature, non-mouldy seeds and good drying of these seeds. We had also noted in Table 6 that the results found during the months of May and June were similar to those found in the laboratory's archive during the fi rst three     months of receipt. This could be explained by the fact that the temperature during these months was favourable for the maintenance of the seeds. The humidity of the environment was acceptable so the temperature was acceptable which did not impact the fat content of the seed. Figure 2 shows that the oil content of the seed, the moisture content and the acidity level were proportional and therefore dependent on each other.

Cotton seed temperature
Both the stored seed and the organisms that attacked it are living, breathing beings. During respiration, oxygen was used and carbon dioxide, water and heat were produced. The amount of carbon dioxide, water and heat produced during respiration depended on the temperature and moisture content of the seed.
In fact, the moisture produced during respiration increased the moisture content of the product while the heat produced increased the temperature. The increases in temperature and humidity created the conditions for the seed to burn. On the other hand, the level of respiration of well-dried cotton seeds at a low moisture content did not cause any increase in temperature. The higher the temperature, the lower the moisture content had to be in order to reduce spoilage and the lower the temperature, the higher the moisture content can be [13].
Temperature, like other factors, had to be controlled during storage to avoid huge seed losses. The studies in [8,[14][15][16][17][18]. showed that this parameter had to be taken into account in order to obtain a quality oil with no effect on human health.

Conclusion
For a seed of very good physical and/or chemical quality, the quality control analyses in the laboratory and on the storage premises have been carried out. The targeted steps are the critical points, as any failure can create hazards that can reach unacceptable limits. The materials used, the method of conservation, checking the humidity of the seed before and during storage, the reserved environment and the internal temperature of the materials during storage are the critical points when storing cotton seed. This study was designed to contribute to the improvement of the storage of the raw material at SN Citec: cotton seed. In view of the results we reached during the analyses on cotton seed, it is clear that the raw material "cotton seed" used by SN Citec is of good quality. Indeed, the moisture, acidity and oil content values analysed during our study complied with the standards of the codex stand. Nevertheless, the monitoring measures must be well applied in order to always guarantee the quality of the seed. In addition to this, the corrective actions implemented must always be respected.The storage of the cotton seed in normal physicochemical condition will minimised losses and breakage during the production of oil.It also allow us to complete on the market the the original qualities of our products and avoid their attack by insects, mold. lot4 20,28 20-24 Table 6: Oil content of cottonseed in different months.