Performance Evaluation of Napier Grass (Penisetum Purpuruem (L.) Schumach) accessions under rain fed and Irrigation System at Wondo Genet

Six Napier grass accessions (Penisetum purpuruem) that have been tested and identifi ed for their better agronomic and yield performance at different national and regional research centers were collected and tested at Wondo genet Agricultural Research center under two sets (under irrigation and rain fed) conditions. The experiment was conducted in randomized complete block design with three replications. Under supplementary irrigation there was no signifi cant difference (P>0.05) of mean yield and yield components observed among accessions both under fertilizer application and without fertilizer application with fresh biomass yield (t/ha) ranged from 43.73 to 70.24 and that of dry biomass yield (t/ha) ranged 11.54 to 20.32. Under rainfed condition the combined mean analysis for tiller number per plant, plant height, node number per plant and internodes length per plant did show signifi cant (P<0.05) while fresh biomass yield and dry matter yield didn’t show signifi cant (P>0.05) difference. Fresh biomass yield (59.11 t/ha) and dry matter yield (16.17 t/ha) for fertilizer application is signifi cantly higher (p<0.05) than fresh biomass and dry matter yield of 40.51 t/ha and 10.51 t/ha respectively for non-fertilizer applied Napier accessions under rain fed condition. Both under supplementary irrigation and rainfed conditions, Napier accessions 14983, 15743 and 16788 responds better mean fresh and dry biomass yield. Hence, among tested genotypes 14983, 15743 and 16788 Napier grass accessions will be encouraged in the study area and similar environment. Research Article Performance Evaluation of Napier Grass (Penisetum Purpuruem (L.) Schumach) accessions under rain fed and Irrigation System at Wondo Genet Aman Getiso* and Diribi Mijena Ethiopian Institute of Agricultural Research, Wondo Genet Agriculral Research Center, P.O. Box 198, Shashemene, Ethiopia Received: 22 April, 2021 Accepted: 28 April, 2021 Published: 29 April, 2021 *Corresponding author: Aman Getiso, MSc, Associate Researcher, Animal Production, Ethiopian Institute of Agricultural Research, Wondo Genet Agriculral Research Center, P.O. Box 198, Shashemene, Ethiopia, Tel: +251911-94-84-11; E-mail: https://www.peertechzpublications.com ORCID: https://orcid.org/0000-0002-2454-1691


Introduction
Livestock production is an integral part of the subsistence crop-livestock mixed farming systems of Ethiopia. The major constraint that infl uences the productivity of livestock is shortage of feed both in quantity and quality [1]. The major feed resources for livestock come from natural pasture and crop residue (Alemu and Lemma 1991). However, they are poor in quality and provide inadequate protein, energy, vitamins and minerals [2].
Nevertheless, the feed supply to animals can be improved by cultivation of tropically adapted forage species, which give reasonable yield under drought and unstable climatic conditions. Among the promising forage species introduced to Ethiopia, Napier grass (Pennisetum purpureum) is reported to be a popular fodder crop in the Ethiopian highlands where it has shown considerable potential to alleviate the severe shortage of high-quality fodder [3]. It is a tall perennial grass, also known as elephant grass, was originated from sub-Saharan tropical Africa and well adapted to the altitude up to 2500m and rainfall 600-1000mm [4] and occurs naturally throughout tropical Africa and particularly in East Africa [5]. It is the forage of choice not only in the tropics but also worldwide due to its desirable traits such as tolerance to drought and adaptability to a wide range of soil conditions and high photosynthetic and water-use effi ciency [6]. Its leafy nature, considerable plant height, high tiller and re-growth ability made it a high productive feed crop per unit area of land as compared to other grass species [7].
Citation: Getiso  Napier grass become ready for harvesting within 3-4 months after planting and harvesting can continue at an interval of 6-8 weeks for more than fi ve years where there is no moisture stress and fertility problem. Napier grass could play an important role in providing a signifi cant amount of biomass yield of 20 to 30 t DM/ha/year with good agronomic and management practices (Farrell et al., 2002). Napier grass is palatable and could be fed fresh, as silage or directly grazed on the fi eld [8,9]. A yield of 85.4 t/ha without fertilizer application and a record high yield of 130 t/ha with 1320 kg/ha of nitrogen fertilizer application have been recorded [10]. Napier grass could be categorized as high-quality forage [11] and extremely palatable when young and leafy [12].
Practices, including fertilization, irrigation and pest control were reported agronomic practices required for production of high yield and quality fodder from Napier grass crops, [13]. Hence the objective of this study was to evaluate the biomass yield and desirable agronomic parameters of selected six Napier grass accessions at Wondo genet Agricultural Research Center under irrigation and rain fed conditions.

Descriptions of the test environments
Under irrigation system the study was conducted at Wando Genet agricultural Research Center which is one of the centers of Ethiopian institute of agricultural research and is found in Sidama Regional state, Wondo Genet woreda. It is situated about 268km south of Addis Ababa and 14 km south east of Shashemene. Its geographical location and altitude ranges from 38 o 37'13''-38o 38'20'' East and 7 o 5'23''-7 o 5'52'' North and 1760-1920 m.a.s.l respectively [14]. The area receives mean annual rain fall of 1128 mm with minimum and maximum temperature of 11 and 26°C, respectively [15].
Set II experiment (under rain fed condition) was conducted at Awada Agriculture Research Sub-center. Awada Agricultural Research Sub-center is situated in the Tepid to cool semi-arid mid highland agroecology. It is located at about 315 km south of Addis Ababa at 603' N of latitude and 380 E of longitude at an altitude of about 1740m a.s.l. nearby Yirgalem town. The area has a semi-bimodal rainfall distribution characterized by double wet and dry seasons with an average precipitation of 1342 mm per annum [16].

Experimental design, layout and treatments
Available accessions of the listed forage grass (Penisetum purpuruem) that have been tested and identifi ed for their better yield and desirable agronomic performance at different national and regional research centers were collected and tested at Wondo genet Agricultural Research center under two sets (under irrigation and rain fed) conditions. The experiment was conducted under fi eld situations for three years during the main cropping seasons of 2010 to 2012 E.C. A 6X2 factorial in a Randomized Completely Block Design (RCBD) was used in performance evaluation of Napier grass accessions study.
There were three replicate plots for each treatment with a total of 36 plots each measuring 10.5m 2 (3.5m*3m). In this trial, Napier grass accessions (14983, 14984, 16808, 16788, 15743, 16819) were designated as one main factor while fertilizer application (with and without fertilizer application) was designed as another factor. Spacing of 0.5 m distance between plots and a 1m distance between replications was used. Each plot was divided into two equal parts to test the performance of tested grass varieties with and without fertilizer application. Fertilizer was applied at the rate 100kg per hectare DAP (46 % P205 and 18 % N) at planting. Plots were hand weeded during the establishment phase.

Harvesting procedure and data collection
The fi rst harvest was at the age of 5 months after planting and then harvested on average of 4.5 months during experimental period. Measurements taken before and after each harvest were number of tillers per plants, node number per plant, Internode's length per plant, plant height and forage DM yield. Plant height was based on fi ve culms taken randomly in each plot, measured using a steel tape from the ground level to the highest leaf. For determination of biomass yield, accessions were harvested at forage harvesting stage from two rows next to the guard rows of 5 to 10 cm above the ground level. Weight of the total fresh biomass yield was recorded from each plot in the fi eld and the estimated 300 g sample was taken from each plot to the laboratory. The sample taken from each plot was weighed to know their sample fresh weight and then oven dried for 24 h at a temperature of 105°C to determine dry matter yield.

Statistical analysis
Differences among accessions were tested using analysis of variance (ANOVA) procedures of SAS General Linear Model (GLM) to compare treatment means [17]. The difference in mean was compared using the Standard Error of the Mean (SEM).

Results and discussion
The mean yield and yield components (Tillering performance, Plant height, Node number per plant, Internode's length per plant, Forage fresh and dry matter yield) for different Napier grass accessions and fertilizers under supplementary irrigation and rainfed was presented in Table 1 and Table 2 respectively. Under supplementary irrigation there was no signifi cant difference (P>0.05) of mean yield and yield components observed among accessions both under fertilizer application and without fertilizer application.
The combined mean number of tillers per plant of each treatment indicated that all Napier grass accessions were not signifi cance difference (P>0.05) among them under rainfed condition with similar effects of fertilizer application (Table  3). There was also no statistically difference (P>0.05) for plant height, node number per plant, fresh biomass yield and dry biomass yield under rainfed condition among Napier grass accessions (Table 3). But statistically difference (P<0.05) internodes length per plant was recorded among Napier grass accessions with least internodes length per plant of accession 15743 (12.9 cm) followed by 16819 (14.94cm) ( Table 3).
Citation: Getiso  Combined analysis of fresh biomass yield (t/ha) ranged from 43.73 to 70.24 and that of dry biomass yield (t/ha) ranged 11.54 to 20.32 (Table 3) under supplementary irrigation condition with no statistically no difference (P>0.05) among Napier accession. The current results for fresh biomass yield and dry biomass yield were higher than the results (22.2 to 57.3 t/ha) and (3.9 to 10.5 t/ha) for fresh biomass and dry biomass yield respectively reported by Habtie et al. [18]. Even though there was no statistically difference (p>0.05) on dry matter yield among Napier grass accessions, the combined dry matter yield for accession 16819 (11.54 t/ha) was in agreement with the result (11.72 t/ha) reported by Kebede, et al. [19] at Debrezeit Location but lower than the results reported by the same author at Hawassa (13.30) and Adamitulu (18.30 t/ha) locations. Yields of the grass vary depending on genotype (Cuomo et al., 1996), edaphic and climatic factors and management practices [20].
The overall combined mean (ranged 13.95 to 21.97 t/ha) dry matter yield of the studied accessions (14983, 14984, 16808,     (Table 4). Fresh biomass yield and dry matter yield (t/ha) is signifi cantly higher (p<0.05) for fertilizer application than for non-fertilizer applied Napier accessions under rain fed condition ( Even though there was no statistically difference (P>0.05) the higher fresh biomass yield (60.28 t/ha) and dry matter yield (16.89 t/ha) was recorded for accession 14983 with higher tiller number per plant under rain fed condition which could be due to the contribution to increase photosynthetic activity and hence higher DM production. The development of new shoots bearing on each plant result in greater number of tillers and increased yield for grasses as the plant matured and increased in tiller density [3]. Tillers number is an important characteristic of grasses as it increases the chances of survival and amount of available forage [21,22].

Number of tiller/plants Plant height (m) Node number per plant Internodes length per plant (cm) Fresh biomass yield (t/ha) Dry matter yield (t/ha)
The plant height in current study under rainfed condition ranged from 2m to 2.66m is higher than the result (ranged 1.07m to 1.26m) reported by Kebede, et al. 2016 [19] at Holeta, Debrezeit, Adamitulu, Hawassa and Areka locations which could be due to different in age at harvesting time. Increments in plant height at later harvest stages could be due to massive root development and effi cient nutrient uptake, letting the plant to bear to increase in height as mentioned by Melkie [23]. Growth characteristics and productivity [24] and fodder yield of Napier grass in Kenya [25] is affected by height at cutting. Amongst the major agronomic practices required, harvesting of Napier grass at appropriate cutting height and defoliation frequencies are very important to improve DM yield and nutritive values of this plant. A higher cutting height of Napier grass may result in underutilization and the quality of forage is reduced by a higher cutting height [26].
Signifi cant (P<0.05) variation was observed among the tested Napier grass accessions under rain fed condition for number of nodes per plant and internodes length per plant (Table 4). In combined analysis under rain fed condition, the number of nodes per plant ranged from 7.38 to 11.65. The mean length of internodes per plant ranged from 12.95 to 17.47. The highest number of nodes per plant was recorded for 16808 (11.65) accession while accession 16819 (7.38) produced the lowest. The highest mean internodes length per plant was recorded for accession 14983 (17.47cm) and the lowest accession was produced by accession 15743 (12.95cm). Generally, as other agronomic traits, stem elongation also infl uenced by variation in soil type, temperature, amount and distribution of rainfall, genotypes and genotype by year interaction effects [27,28]. Under rainfed condition, the mean yield and yield component for Napier grass accessions with fertilizer application is signifi cantly (p<0.05) higher than those accessions not applied with fertilizer (Table 2). This could be due to Napier grass is  (Table 3). This could be attributed with the good soil fertility condition of the experimental site Table  4.