Some “Biological Nonsenses” from the position of the karyogamic theory of carcinogenesis

Malignant transformation of the normal somatic cells into tumorous ones can be assisted by the very difference in their nature agents and factors: penetrating radiation of different nature, numerous chemical substances, some oncogenic and infectious viruses, some toxins, pharmacological agents, some kind of irritation and some non-carcinogenic substance. Probably, the above-numerated agents and factors start up some a common mechanism of the conversion of normal cells into transformed state. As shown, carcinogenic potencies of some fatty acids, carbohydrates, and even distilled water and saline may be explained from the standpoint of karyogamic theory of carcinogenesis. All above-mentioned substances may induce perforations of different volume of plasma membranes of somatic cells. This may premise for cells’ fusion process. In result of perforations, total negative charge of plasma membranes decrease, and the somatic cells acquire the capability of contacts with each other, which upon coincidence of the perforated sites of these organoids, may serve as a prerequisite to fusion process. Review Article Some “Biological Nonsenses” from the position of the karyogamic theory of carcinogenesis Gogichadze GDK*, Gedenidze SG and Gogichadze TG Medical Microbiologist, Molecular Biology and Parasitology, Tbilisi State Medical University, Georgia Received: 15 November, 2019 Accepted: 29 June, 2020 Published: 30 June, 2020 *Corresponding author: Gogichadze GDK, Medical Microbiologist, Molecular Biology and Parasitology, Tbilisi State Medical University, Georgia, Tel: +995 593 54 80 50; E-mail: ORCID: https://orcid.org/0000-0001-5355-0848

Explanation of these strange facts by means of already existing different hypotheses and theories of carcinogenesis is impossible. In this article we make attempt to explain the essence of this occurrences by means of karyogamic theory of carcinogenesis [1][2][3][4].
Citation: Gogichadze  At the same time, some scientists report on fusogenic effect of different (but not all!) fatty acids in in vitro tissue culture [12][13][14]. In these scientifi c works numerous fatty acids were studied on their fusogenic properties. These scientists concluded that easiest fusion process occurs with unsaturated fatty acids.
The mechanism inducing malignant neoplasms in human by these substances is so far unknown. Explanation of this mechanism on the basis of such famous modern theories of carcinogenesis, as polyetiologic, virus-genetic, mutation, oncogenes an so on, is impossible. But parallelism between carcinogenicity and fusogenicity of these substances, allow us to consider this phenomenon as confi rmation of somatic hybridization theories of carcinogenesis.
On the basis of world literature data and our own theoretical and experimental observations [15,16] we attempt to explain the carcinogenicity of some dietary fats from the standpoint of hybridization theory of carcinogenesis (in particular, from karyogamic or two synkaryons theory).
In our experiments it was established that polyunsaturated (linoleic) and monounsaturated (oleic) fatty acids differ from saturated acids by the high intensity interaction with the cellular membranes. Especially high fusogenic activity has oleic acid. But by means of epidemiological analysis carcinogenic activity of this fatty acid was not established. What is the reason of this strange occurrence? Reverse correlation between fusogenic and carcinogenic abilities was revealed: higher is the fusogenic activity of this or that substance, lower is its' carcinogenic activity and vice versa. In particular, in case of high fusogenic ability of the substance (for example, oleic acid) formation of giant unviable polykaryocytes was induced and carcinogenic effect was less manifested; and vice versa, carcinogenic effect was higher in the presence of low fusogenic ability of the substance (for example, linoleic acid) because of formation of mainly dikaryons with high oncogenic potency [15,16].
Some dietary fats may induce perforations of defi nite size of somatic cells in several conditions. This may be premise for cells' fusion and hetero-and homokaryon formation.
Presumably, during the perforation of a cellular membranes, the total negative charge of plasma membranes decreases, and the somatic cells acquire the capability to approach closer (adhesion), which frequently, especially, upon coincidence of the perforated parts, may serve as a prerequisite to fusion process.

Induction of malignant tumors by distilled water
As was shown after experimental works of Warabioka [28,29], Watanabe and his co-authors [30], the daily inoculation of distilled water into intraperitoneal cavity of rats, in some cases development of connective tissue malignant tumors -sarcomas, in particular, fi brosarcomas, maybe induced. For instance, in experiments of Watanabe, in 2 rats after daily inoculation of 3 ml of distilled water, during 15-17 months fi brosarcomas were observed, which in one case was transplantable. Warabioka, in 13 months after daily injection of distilled water, in 1 rat observed sarcoma, which at lethal stage reached the size of a hen's egg. Inoculation of 1%, 1,5% or 2% aqueous solution of trypan blue injected subcutaneously in the Wistar or Sprague-Dawley rats (0,5 ml per 150 g body weight, fortnightly) induced production of hepatic sarcomas [30].
Interpretation of these results from the position of different theories and hypotheses of carcinogenesis, turn out to be impossible. For example, it is diffi cult to express, how distilled water can activate some endogenous viruses or induce expression of defi nite oncogenes with the following development of malignant neoplasms.
At fi rst sight, it is complicated to explain these events from the position of karyogamic theory too, such as the fusogenic properties of distilled water is yet unknown.
Confi rmation of the above-mentioned may be a data of Sokolov [31], Perishing of considerable quantity of somatic cells in result of plasma membranes destruction directly on site of injection, as known, may be favorable for the process of regeneration of corresponding tissue and intensive proliferation of cells (by production of "growth factor"). This in its' turn may involve the process of precancerous synkaryons proliferation too.
Precancerous cells after some processes on molecular and subcellular levels may be transformed into tumor synkaryons.

Induction of malignant neoplasms by saline
As it is known, several data of scientifi c literature testify Distilled water and saline has capacity for destroying somatic cell's plasma membranes. Perforations of these organoids are the reason of cellular membranes' fusion. Daily injection of distilled water and saline into laboratory animals may induce perforations of plasma membranes of somatic cells, which assist their interaction, following a fusion process.