ISSN: 2641-3027
Archive of Biomedical Science and Engineering
Research article       Open Access      Peer-Reviewed

Mycotoxins produced by Fusariumspecies associated with maize ear rot in Iran

Maryam Fallahi and Hossein Saremi*

Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
*Corresponding author: Hossein Saremi, Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran, E-mail: hsn.saremi@ut.ac.ir
Received: 03 June, 2019 | Accepted: 02 September, 2020 | Published: 03 September, 2020
Keywords: Fusarium; maize ear rot; HPLC; Toxigenic risk

Cite this as

Fallahi M, Saremi H (2020) Mycotoxins produced by Fusariumspecies associated with maize ear rot in Iran. Biomed Sci Eng 6(1): 034-038. DOI: 10.17352/abse.000018

Mycotoxins contamination is one of the most important problems worldwide in maize that can cause serious threat for human and animal health. The aim of this study was to determine the ability of Fusariumspecies associated with maize ear rot to produce diverse mycotoxins. The results showed, One out of three isolates of F. subglutinans produced detectable level of Beauvericin (BEA); the only isolate of F. temperatum produced 302 µg/g of BEA. Two out of five isolates of F. redolens produced enniatin B1and four isolates of this species produced high levels of BEA. As well, 21 isolates of Fusariumincarnatum-equiseti species complex (FIESC) and one isolates of F. brachygibbosum were evaluated for production of trichothecens (T-2 toxin, HT-2 toxin, Diacetoxyscirpenol (DAS), nivalenol (NIV) and deoxynivalenol (DON)), zearalenone (ZEN), enniatins (ENN A, A1, B and B1) and BEA by HPLC. Production of trichothecens, ZEN and BEA with one, three and four isolates of FIESC, respectively were observed and one isolate of F. brachygibbosum only produced detectable level of DAS and ENN B1. Analyzing of moniliformin production of F. proliferatum (26 isolates), FIESC (21 isolates) and F.thapsinum (10 isolates) showed none of them produce this toxin. These results revealed the ability of Fusariumspp. from maize to produce a varied range of mycotoxins which are harmful to human and animal’s health. Therefore, the occurrence of such broad number of different species on Iranian maize could be reason of great concern because of the toxigenic risk associated to these species.

Introduction

Mycotoxins have significant influence in food and feed safety. Challenges in mycotoxin and toxigenic fungi research are still enormous, due to the frequency. Maize ear rot is one of the serious diseases caused by Fusariumspp. that significantly reduces the quantity and quality of maize [1,2]. Each Fusariumspecies has its own mycotoxin profile. As a consequence, mycotoxin contamination in maize kernels from fields and silos is often high [3,4]. Fumonisins (FBs), Zearalenone (ZEN) and trichothecenes (Type A: T-2 and HT-2 toxins and Diacetoxyscirpenol (DAS); type B: Deoxynivalenol (DON) and Nivalenol (NIV)) are the most important classes of mycotoxins that are produced by Fusariumspecies. Besides, Fusariumgenera produce emerging mycotoxins such as fusaproliferin (FUS), beauvericin (BEA), enniatins (ENNs) and moniliformin (MON), which have been recently discovered and less studied [5,6]. Fumonisins have been related to several animal and human diseases such as esophageal cancer (EC), as reported in several countries worldwide [7]. A recent study on the fungal mycoflora associated with maize kernels in some maize-producing areas of Iran revealed that F. verticellioides and F. proliferatum are prevalent species in Iranian maize [8-10], since they are both the main producing species of the carcinogenic fumonisins in maize [8]. Moreover, there are other reports in the literatures on the Fusariumincidence, FBs production and levels of fumonisin in Iranian maize and maize –based products [11-13] . Trichothecenes is other important group of Fusariumtoxins related to several livestock diseases. There is poor information about these toxins and related fungi on maize in Iran. On the other hand, ZEN is an estrogenic mycotoxin produced by several species of Fusarium. A high risk of ZEN-contaminated maize for animal and human health was also reported in Iran [14-17]. Since maize is an important cereal crop in Iran and worldwide, its quality and safety is of major concern. Hence, evidence about mycotoxin profiles of Fusariumspecies as an important pathogen of this crop can help us in the development of strategies to reduction of mycotoxin production and prediction the presence of them in maize. Therefore This work is aimed to assess the mycotoxin production of Fusariumisolates from Iranian maize.

Materials and methods

Sampling and fungal isolation

Maize samples of two crop seasons, 2015 and 2016, were collected in September–October from fields and maize grain silos of ten provinces of Iran: Khuzestan, Fars, Golestan, Ardabil, Alborz, Qazvin, Zanjan, Kermanshah, Lorestan and Isfahan. The sampling method in every field was based on hierarchical method [18]. Kernels were surface sterilized for 1 min in 3% sodium hypochlorite solution, rinsed twice in sterile distilled water, dried on filter paper and placed on petri dishes containing Nash & Snyder medium (1 L of distilled water, 15 g of peptone, 1 g of K2HPO4, 0.5 g of MgSO4.7 H2O, 15 g of agar, 1 g of PCNB (Terraclor 75% WP) (pentacloronitrobenzene)). Petri dishes containing kernels were incubated at 25 °C in the dark for 5–7 days. All the developed cultures from the kernels were transferred to potato dextrose agar (PDA) using a single-spore technique and incubated at 25 °C for 7 days. Morphological and molecular identification was done based on methods previously used by Fallahi, et al. [8].

Mycotoxins analysis
In vitro mycotoxin production and toxicity

Selected 57 Fusariumisolates, representative of the Fusariumspecies belonging to F.proliferatum (26 isolates), F. subglutinans (three isolates), F. temperatum (one isolates), Fusariumincarnatum-equiseti species complex (FIESC) (21 isolates), F. brachygibbosum (one isolates), F.redolens (5 isolates) were examined for mycotoxin production.

For mycotoxin production assays, we used 30 g rice in PYREX Glass Erlenmeyer Flasks, added with 13.5 ml of distilled water, standing overnight, and then autoclaved at 121 °C for 30 min. The flasks containing autoclaved rice were inoculated with piece of fungal cultures grown on PDA and incubated at 25 °C for 21 days in order to allow fungal development and mycotoxin production. High-Performance Liquid Chromatography (HPLC) was used to detect mycotoxins trichothecenes, ZEN , BEA and ENNs. Standards of these toxins were obtained from Sigma Aldrich (Milan, Italy) and stored at 4° C in darkness.

Determination Zearalenone (ZEN), beauvericin (BEA), enniatins (ENNA, ENNA1, ENNB and ENNB1) and Moniliformin (MON) production. ZEN production was analyzed for FIESC (21 isolates) and F. brachygibbosum (one strain). In the same way, FIESC (21 isolates), F. brachygibbosum (one strain), F. redolens (five isolates), F.subglutinans (three isolates), and F.temperatum (one isolate) were assayed for BEA and ENNs production. As well, production of MON for F. proliferatum (26 isolates), F.incarnatum-equiseti species complex (21 isolates) and F.thapsinum (10 isolates) was analyzed. One gram of inoculated rice culture was used for toxin extraction with 5 mL of methanol/water (70: 30, v/v). Samples were placed for 60 min in an orbital shaker, and then was filtered using Whatman no. 4 filters (Maidstone, UK). The sample (100 µL) was diluted with 900 µL ultrapure water (Millipore, Bedford, MA) and filtered using RC through 0.20 µm regenerated cellulose filter (Phenomenex, Torrance, CA, USA). A volume of 100 µL of extract was injected into HPLC apparatus (Agilent 1260 Series, Agilent Technology, Santa Clara, CA, USA) that equipped with a binary solvent manager. The analytical column was a Gemini (150 x 4.6 mm, 5 μm, Phenomenex) preceded by a Security Guard™ cartridge Gemini (4 x 3 mm, Phenomenex). Retention time of the ZEN was about 7.8 min, 11.4 min for BEA, 9 min for ENN B, 10.3 min for ENN B1, 12 min for ENN A1 and 13 min for ENN A. MON respectively was evaluated according to the analysis method of BEA and ENN with a slight change. The retention time of MON was about 2.7 min.

Determination of Trichothecenes group A (T-2, HT-2 toxins and Diacetoxyscirpenol (DAS)) and group B (nivalenol (NIV) and deoxynivalenol (DON)).

FIESC (21 isolates) and F. brachygibbosum (one isolates) were evaluated for production of trichothecenes group A (T-2, HT-2 toxins and DAS) and group B (NIV and DON). Then 1 g of rice culture that inoculated with fungi was used for extraction of toxin with 5 mL of acetonitrile/water (84:16, v/v) with 1 % of acetic acid by orbital shaking for 2 h. After filtration through Whatman no. 4 filters (Maidstone, UK), 100 µL of sample was diluted with 900 µL ultrapure water. The residue was filtered using RC through 0.20 µm regenerated cellulose filter. Finally, 10 µL of extract was injected into to HPLC apparatus (Agilent 1260 Series) for trichothecenes group A and 50 µL for group B. The analytical column was a ZORBAX Eclipse Plus C18 (50 mm × 2.1 mm i.d., 1.8 μm) for group A and Synergi Hydro-RP 80A (150 x 3 mm, 4 μm, Phenomenex) for group B. The retention time of the HT-2 was about 1.97 min, 4.9 min for T-2, 14.6 min for DAS, 8.2 min for DON and 4.1 min for NIV.

Results

Mycotoxin production

The mycotoxin production of isolates is reported in Tables 1 and Table 2. In Table 1 the values of the ENNs and BEA production by F. subglutinans, F. temperatum and F. redolens isolates are reported. As well, In table 2 the values of the Trichothecenes, ZEN, ENNs and BEA production by FIESC and F. brachygibbosum isolates are described.

One of three isolates of F. subglutinans could produce detectable level of BEA. The only isolate of F. temperatum produced 302.1 µg/g of BEA. Two out of five isolates of F. redolens produced detectable level of ENN B1. As well, BEA production was detected by four isolates of F. redolens varied from 688 µg/g to 7936 µg/g (Table 1).

The results showed that only one isolate of FIESC can produce each of T-2, HT-2 toxin and DAS. Three and four isolates of FIESC produced ZEN and BEA respectively. One isolate of F. brachygibbosum produced detectable level of DAS and ENN B1. None of these 22 isolates produced NIV, DON, MON, ENN A, ENN A1 and ENN B1 (Table 2).

In other part, the moniliformin production of F. proliferatum (26 isolates) and F.thapsinum (10 isolates) was evaluated and none of them produces this toxin.

Discussion

Due to the importance of quality and quantity of maize as a significant source in human and animal nutrition, we evaluated the Fusariummycotoxin production in the maize-producing areas in Iran. Most of the studies have emphasized on “traditional” mycotoxins, such as aflatoxins, ochratoxin A and trichothecenes, ZEN and fumonisins. However, Fusariumspp. is also capable of producing other toxic secondary metabolites the so-called emerging mycotoxins such as beauvericin, enniatins, and moniliformin. So far, only limited data is available on these metabolites. This is not only due to their late recognition but especially the late understanding of their role as mycotoxins [19].

F. temperatum was reported from maize in several studies; but formerly, there was no report of occurrence of it on Iranian maize. This species has potential to produce diverse mycotoxins among which beauvericin and enniatins [20-22]. Previous studies [23] reported the BEA production by F. subglutinans isolates from different geographical areas, but this contrast with further report [6] was due to the identification of F. temperatum as a different species than F. subglutinans meanwhile. Afterwards [24,25] showed that F. temperatum and F. subglutinans can be distinguished based on the BEA production. In our study the only isolate of F.temperatum produced high level of BEA while only one of three isolates of F.subglutinas produced low level of this toxin. Certainly being able to analyze more isolates of F.subglutinans and F.temperatum from Iran, it could be possible to give detailed information about their mycotoxin profile. A species very closely related to Fusariumfujikuroi species complex (FFSC) is Fusariumredolens, formerly identified as F. oxysporum. All F. redolens analyzed isolates produced ENNs and high level of BEA. ENN and BEA have demonstrated not only toxicity on human cell lines, but also on other models. BEA is structurally similar to the ENN and BEA, but BEA differs in the nature of the N-methylamino acid. Owing to this difference between BEA and the ENN, their bioactivities are obviously different. BEA has antimicrobial and anti-tumor activities. Also, antibacterial activity and antifungal capacity of ENN were demonstrated [26,27]. Based on the EF-1α gene sequences, 21 isolates were identified as belonging to FIESC, a complex of 30 phylogenetic species [28,29] and one isolate as F. brachygibbosum, a closely related species. Previously only few studies reported FIESC isolates from maize in, India and Malaysia [30,31]. FIESC reported as specially trichothecenes and a number of other mycotoxins, such as butenolide, beauvericin, equisetin, fusarochromanone and zearalenone producer (Desjardins, 2006). We analyzed the ability of 21 strains of FIESC to produce different mycotoxin and only few isolates of this species could produce each of T-2, HT-2 toxin, DAS, ZEN and BEA with no production for NIV,DON, ENN and MON.

Previous literatures referred to production of MON by F.proliferatum and F.thapsinum [32,33] but none of these two species produced MON in this research. The results obtained in the present study showed that, the mycotoxin production of the isolates from different regions was varied and there was no significant relationship between mycotoxins production and geographic origins of isolates.

Although distribution of mycotoxins in different regions depended on several factors such as environmental conditions, endogenous and exogenous factors, the analysis of mycotoxin production in vitro can be useful in forecast the mycotoxins contamination in the field [34]. So, determination the mycotoxin profiles of the Fusariumspecies, as the most common toxigenic species on maize, provide useful elements to evaluate the potential risk of mycotoxin contamination in Iranian maize. These results are beneficial for more efficient management of these pathogens in fields and silos.

We thank the University of Tehran and for providing facilities for doing this research.

  1. Gallo A, Giuberti G, Frisvad JC, Bertuzzi T, et al. (2015) Review on mycotoxin issues in ruminants: occurrence in forages, effects of mycotoxin ingestion on health status and animal performance and practical strategies to counteract their negative effects. Toxins, 7: 3057-3111. Link: https://bit.ly/3bkBYdj
  2. Stoev SD (2015) Foodborne mycotoxicoses, risk assessment and underestimated hazard of masked mycotoxins and joint mycotoxin effects or interaction. Environ Toxicol Pharmacol 39: 794-809. https://bit.ly/31NOor5
  3. Aguín O, Cao A, Pintos C, Santiago R, Mansilla P, et al. (2014) Occurrence of Fusariumspecies in maize kernels grown in northwestern Spain. Plant pathology 63: 946-951. https://bit.ly/3bm3Ygx
  4. Alizadeh AM, Roshandel G, Roudbarmohammadi S, Roudbary M, Sohanaki H, et al. (2012) Fumonisin B1 contamination of cereals and risk of esophageal cancer in a high risk area in northeastern Iran. Asian Pac J Cancer Prev 13: 2625-2628. https://bit.ly/3bjaCUZ
  5. Escrivá L, Font G, Manyes L (2015) In vivo toxicity studies of Fusariummycotoxins in the last decade: a review. Food and Chemical Toxicology 78: 185-206. Link: https://bit.ly/2EIJSBA
  6. Moretti A, Mule G, Ritieni A, Logrieco A (2007) Further data on the production of beauvericin, enniatins and fusaproliferin and toxicity to Artemia salina by Fusariumspecies of Gibberella fujikuroi species complex. Int J Food Microbiol 118: 158-163. https://bit.ly/34VozXU
  7. Desjardins AE (2006) FusariumMycotoxins: Chemistry, Genetics, and Biology; American Phytopathological Society (APS Press): St. Paul, MN, USA.
  8. Fallahi M, Saremi H, Javan-Nikkhah M, Somma S, Haidukowski M, et al. (2019) Isolation, Molecular Identification and Mycotoxin Profile of Fusarium Species Isolated from Maize Kernels in Iran. Toxins11: 297. Link: https://bit.ly/31QLO3F
  9. Ghiasian SA, Kord-Bacheh P, Rezayat SM, Maghsood AH, Taherkhani H (2004) Mycoflora of Iranian maize harvested in the main production areas in 2000. Mycopathologia 158: 113-121. Link: https://bit.ly/3gWab3X
  10. Zamani-Zadeh HR, Khorsandi H (1995) Occurrence of Fusariumspecies and their mycotoxins in wheat in Mazandaran province. Iranian Journal of Plant Pathology 31: 12-14. https://bit.ly/31Qkmmr
  11. Amirahmadi M, Shoeibi S, Rastegar H, Elmi M, Mousavi Khaneghah A (2017) Simultaneous analysis of mycotoxins in corn flour using LC/MS-MS combined with a modified QuEChERS procedure. Toxin Reviews 37: 1-9. https://bit.ly/3gZybnb
  12. Ghiasian SA, Maghsood AH, Yazdanpanah H, Shephard GS, Van Der Westhuizen L, et al. (2006) Incidence of Fusariumverticillioides and levels of fumonisins in corn from main production areas in Iran. J Agric Food Chem 54: 6118-6122. https://bit.ly/3bkCRTb
  13. Yazdanpanah H, Shephard S, Marassas WFO, Brown NL, Rahimian H, et al. (2000) Natural occurrence of fumonisin in corn from the Mazandaran and Isfahan provinces. Proc. 14th Iranian Palnt Protec Cong 45. J Agric Food Chem 48. Link: https://bit.ly/3bjYB1p
  14. Rashedi M, Sohrabi HR, Ashjaazadeh MA, Azizi H, et al. (2012) Zearalenone contamination in barley, corn, silage and wheat bran. Toxicol Ind Health 28: 779-782. Link: https://bit.ly/2QPhPml
  15. Nuryono N, Noviandi CT, Böhm J, Razzazi-Fazeli E (2005) A limited survey of zearalenone in Indonesian maize-based food and feed by ELISA and high performance liquid chromatography. Food control 16: 65-71. https://bit.ly/2YXMNgl
  16. Oveisi R, Hajimahmoodi M, Memarian S, Sadeghi N, Shoeibi S (2005) Determination of zearalenone in corn flour and a cheese snack product using high-performance liquid chromatography with fluorescence detection. Food additives and contaminants 22: 443-448. https://bit.ly/34V1bd5
  17. Hadiani MR, Yazdanpanah H, Ghazi-Khansari M, Cheraghali MA, et al. (2003) Survey of the natural occurrence of zearalenone in maize from northern Iran by thin-layer chromatography densitometry. Food Addit Contam 20: 380-385. Link: https://bit.ly/3hU9dql
  18. McDonald BA, Zhan J, Burdon JJ (1999) Genetic structure of Rhynchosporium secalis in Australia. Phytopathology 89: 639-645. https://bit.ly/2Z0hX73
  19. Jestoi M (2008) Emerging Fusarium-Mycotoxins Fusaproliferin, Beauvericin, Enniatins, And Moniliformin—A Review, Critical Reviews in Food Science and Nutrition 48: 21-49.
  20. Wang JH, Zhang JB, Li HP, Gong AD, Xue S, et al. (2014) Molecular identification, mycotoxin production and comparative pathogenicity of Fusarium temperatum isolated from maize in China. Journal of Phytopathology 162: 147-157. Link: https://bit.ly/2Z0ulDX
  21. Varela CP, Casal OA, Padin MC, Martinez VF, Oses MS, et al. (2013) First report of Fusariumtemperatum causing seedling blight and stalk rot on maize in Spain. Plant Disease 97: 1252-1252. Link: https://bit.ly/3gYpz04
  22. Summerell BA, Leslie JF (2011) Fifty years of Fusarium: how could nine species have ever been enough?. Fungal Diversity 50: 135. Link: https://bit.ly/3bigNZv
  23. Moretti A, Logrieco A, Bottalico A, Ritieni A, Randazzo G, et al. (1995) Beauvericin production by Fusariumsubglutinans from different geographical areas. Mycological Research 99: 282-286. Link: https://bit.ly/2EZjByo
  24. Moretti A, Mulé G, Ritieni A, Láday M, Stubnya V, et al. (2008) Cryptic subspecies and beauvericin production by Fusariumsubglutinans from Europe. Int J Food Microbiol 127: 312-315. Link: https://bit.ly/3bnP1ee
  25. Scauflaire J, Gourgue M, Callebaut A, Munaut F (2012) Fusarium temperatum, a mycotoxin-producing pathogen of maize. European Journal of Plant Pathology 133: 911-922. Link: https://bit.ly/31RdlBP
  26. Wang Q, Xu L (2012) Beauvericin, a bioactive compound produced by fungi: a short review. Molecules 17: 2367-2377. Link: https://bit.ly/3hRmFLz
  27. Blesa J, Marín R, Lino CM, Mañes J (2012) Evaluation of enniatins A, A1, B, B1 and beauvericin in Portuguese cereal-based foods. Food Additives & Contaminants: Part A 29: 1727-1735. Link: https://bit.ly/32SKcFX
  28. O'Donnell K, Sutton DA, Rinaldi MG, Gueidan C, Crous PW, et al. (2009) Novel multilocus sequence typing scheme reveals high genetic diversity of human pathogenic members of the Fusariumincarnatum-F. equiseti and F. chlamydosporum species complexes within the United States. Journal of Clinical Microbiology 47: 3851-3861. Link: https://bit.ly/34YvWOy
  29. O'Donnell K, Humber RA, Geiser DM, Kang S, Park B, et al. (2012) Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and FusariumMLST. Mycologia 104: 427-445. Link: https://bit.ly/3jzNDbd
  30. Aiyaz M, Divakara ST, Mudili V, Moore GG, Gupta VK, et al. (2016) Molecular Diversity of Seed-borne FusariumSpecies Associated with Maize in India. Current Genomics 17: 132–144. https://bit.ly/3lHSk4I
  31. Zainudin NAIM, Sidique SNM, Johari NA, Razak AA, Salleh B (2011) Isolation and Identification of FusariumSpecies Associated with FusariumEar Rot Disease of Corn. Pertanika Journal of Tropical Agricultural Science 34: 2. https://bit.ly/3bkD3C7
  32. Leslie JF, Zeller KA, Lamprecht SC, Rheeder JP, Marasas WF (2005) Toxicity, pathogenicity, and genetic differentiation of five species of Fusariumfrom sorghum and millet. Phytopathology 95: 275-283. Link: https://bit.ly/3gZA8A1
  33. Marasas WFO, Thiel PG, Rabie CJ, Nelson PE, Toussoun TA (1986) Moniliformin production in Fusariumsection Liseola. Mycologia 78: 242-247. Link: https://bit.ly/31S5N1P
  34. Ferrigo D, Raiola A, Causin R (2016) Fusariumtoxins in cereals: occurrence, legislation, factors promoting the appearance and their management. Molecules 21: 627. Link: https://bit.ly/3500sHE
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