Morphometry of the Corpus Callosum

Corpus callosum (CC) is the largest fi ber pathway linking the two cerebral hemispheres of the brain. These connections through the CC are either homotopic that connect the same or similar areas on each hemisphere or heterotopic that connect functionally similar, but anatomically different areas in two hemispheres. That means it plays an important role in integration and communication of hemispheres. As having morphological differences among people, being a structure that completes its myelinization later and because of its functional importance, it appeal to researchers. The aim of this review was to evaluate the functional anatomy of the CC. By the use of tractographies and functional MRIs, topographic organization of CC and the effect of neurodevelopmental and neurodegenerative processes may be well understood. Mini Review Morphometry of the Corpus Callosum Aysegul Firat1* and Fadime Irsel Tezer Filik2 1Hacettepe University Faculty of Medicine Department of Anatomy, Ankara, Turkey 2Hacettepe University Faculty of Medicine Department of Neurology, Ankara, Turkey Dates: Received: 16 December, 2016; Accepted: 29 December, 2016; Published: 30 December, 2016 *Corresponding author: Aysegul Firat, Hacettepe University Faculty of Medicine Department of Anatomy, Ankara, Turkey, Tel: +90(312)3052132; Fax: +90(312)3107169; E-mail:


Introduction
The corpus callosum (CC) is the largest interconnection between the brain hemispheres. The interhemispheric connections through the CC may connect functionally same or similar areas in the hemispheres. Nowadays by the help of the higher imaging techniques it has been shown that these fi bers exhibit a topographic organization [1]. In the literature CC is examined in detailes according to it's dimensions, parts, morphology, age and gender differences and also according to the changes related to the neuropsychiatric diseases [1][2][3][4][5][6][7]. It has now been accepted that the morphological change of the brain development is affected by growth, development, aging, environmental factors and diseases [8,9]. The CC is one of the very important structures that is affected by these factors and these changes appeal to researchers [1][2][3][4]. In this review, we will investigate the signifi cant studies related with the morphological differences and changes of the CC.
In classical anatomy textbooks [10], CC is divided into fi ve parts: rostrum, genu, body, isthmus and splenium. Fibers of the CC have a topographical organisation based on structural connectivity. Map of these cortical tracts within the CC can be obtained by in vivo imaging techniques [1,11]. But borders of the topographies, number and diameter of the fi bers in each tract, accurate route of the fi bers cannot be evaluated by now. These topographical maps might help us to understand the effects of damage on CC in neurodevelopmental and neurodegenerative diseases.
Increased total callosal area would be because of myelination, fi ber thickness, increased density of fi bers and increased number of fi bers. When we consider the development of CC, it has been established that there was not an increase in the number of fi bers after birth. Controversial to that, during this period there has been a decrease in the number of axons because of some regressive reasons. This developmental callosal analysis was declared by Koppel and Innocenti [12], by a quantitative electron microscobic study in the cat and by LaMantia and Rakic [13], in the developing rhesus monkey.
According to these studies during the early postnatal life, in both rhesus monkey and cat, there is a loss of callosal fi bers; lasting at least for 3 or 4 months postnatally. Now we know that this process might extend beyond the fi rst year of life in human [8,9,14].
Especially by the developing imaging techniques like functional magnetic resonance imaging (fMRI), many studies related with the fi ber organisation and functional anatomy of the CC have been produced. Zarei et al. [1], used diffusion tensor imaging (DTI) tractography to produce a two-dimensional map of the CC of 11 right-handed healhty subjects in the midsagittal plane. By using DTI tractography they observed an antero-posterior topography of interhemispheric connections within the CC and compared volumes between hemispheres. In this study, cortical connections were successfully traced within CC in all subjects and they found the antero-posterior locations of the connections: connections of the prefrontal cortex were located within the genu and anterior part of the body; premotor followed by motor and sensory connections were located in the midbody region; parietal, temporal and occipital connections were located at posterior parts of the CC in orderly ( Figure   1). They have found no effect of hemisphere and gender for absolute CC volume. Wahl et al. [11], also have found results that are in agreement with other previous DTI studies examining  Witelson [2], has studied the hand and sex differences in the human CC in a postmortem morphological study. In this study, she has subdivided the midsagittal surface of the callosum into seven regions, roughly approximating the topography of callosal fi bres in relation to their cortical fi elds of origin and termination. This possible functional organisation has been supported by many animal and clinical experiments previously and also by developing imaging techniques like fMRI nowadays [1,[15][16][17][18][19]. Witelson [2] was measured midsagittal area of the CC in 50 autopsy brains. They found sex differences in many aspects of CC anatomy: In almost all studies males were tended to have larger callosal sizes [2][3][4]. Whether there are gender differences in the size or the morphology of the CC is still a question. In spite of all these discussions; De Lacoste-Utamsing and Holloway [20], have measured the midsagittal callosal area in 14 postmortem brains and they found that females had greater CC.
This difference was prominent in splenium and they explained the situation as a result of the cognitive and speech abilities of women. Holloway and De-Lacoste [21], have repeated the same study (n=16) and they have found that genu, anterior body and total area of CC were greater in men and isthmus was greater in women when compared with right-handed men.
Hand preference did not make any difference among women and there was a decrease in size in men with aging. But in both studies number of the groups were very small and this was decreasing the valubility of the results.
Berrebi et al. [22], studied total callosal area, perimeter and thickness of the CC in newborn rats. They found that all measurements were greater in male brains. One group of rats was handled 110 days, other group was handled for 215 days in the same study and the results were compared with a control group. At 110 days handling stimulation increased callosal parameters and resulted in a more regular CC in males, but this effect was no longer apperent in 215 days. Within the CC region specifi c effects were found, suggesting that certain fi bers of CC were involved. It has been shown that animals given extra stimulation in early life have more lateralized brains [23,24]; thus at least in this experiment, increased callosal size and regularity is associated with greater hemispheric specialisation.
Denenberg et al. [23,24] has postulated in his study that handling stimulation increases the size of the CC and this increase means more competition between the hemispheres which result in laterality.
All these studies provide evidence that the morphometry of CC is important in the diagnosis of many neurological diseases.   It has been discussed that these measurements might also be related to with the impairment of functional interactions both within each hemisphere and between the two hemispheres.
The degree of the impairment diminishes the cognitive, verbal and executive performances. In the clinical trials normal and pathological developmental processes and effects of some diseases on these processes very striking subjects. White matter is essential for impulse conduction, for that reason the white matter plasticity widens the area of research beyond the electrophysiology in the absence of pathology. But much work needs to be done to explore these interesting networks. This includes determining the nature of the white matter structural changes in both normal processes and in the pathological processes in the next years. Imaging and cellular and molecular modalities reveal that white matter changes with possible functional and psychological disorders are important for the normal CC function and for the clinical conditions.