Open Access Research Article Article ID: OJPS-1-103

    Modulation of the Activity of Enzymes Involved in Carbohydrate Metabolism during Flower Development of Grapevine ( Vitis Vinifera L. )

    Cédric Jacquard*, Gaël Lebon, Marine Rondeau, Lisa Sanchez, Essaid Ait Barka, Nathalie Vaillant-Gaveau, Christophe Clément

    In order to further understand the relationships between flower development and sugar metabolism in grapevine, the fluctuations of both starch and sucrose contents were compared with the activity of their related enzymes, in the inflorescences, from the appearance of flower buds until the fruit set. The measurements were carried out on GW and PN cvs., differing in their sensitivity to the flower abscission. The meiosis stage, which is a crucial step for the achievement of sexual reproduction, was particularly screened. Results indicate that the main differences in carbohydrate metabolism occur during meiosis. In the inflorescences of both cvs., variations of enzyme activities can be correlated with their differences in sugar contents. Starch fluctuations were mediated by the activity of amylases (alpha- and beta-) rather than by starch synthase. Changes of sucrose were correlated with the activity of Starch Synthase degradation, both cytoplasmic and wall-bounded invertases but not with the Sucrose Phosphate Synthase activity. Finally, the significant increase of sucrose degrading enzyme activities, such as Starch Synthase degradation, cytoplasmic invertase, and wall-bound invertase, observed after the flower separating stage was interpreted as the first sign of the strong physiological modifications occurred in the ovaries between fertilization and the fruit formation.

    Keywords: Carbohydrate metabolism; Enzyme activities; Grapevine; Inflorescences; Starch; Sucrose

    Published on: Oct 14, 2016 Pages: 10-17

    Full Text PDF Full Text HTML DOI: 10.17352/ojps.000003
    CrossMark Publons Harvard Library HOLLIS Search IT Semantic Scholar Get Citation Base Search Scilit OAI-PMH ResearchGate Academic Microsoft GrowKudos Universite de Paris UW Libraries SJSU King Library SJSU King Library NUS Library McGill DET KGL BIBLiOTEK JCU Discovery Universidad De Lima WorldCat VU on WorldCat


    Global Views

    Case Reports

    Peertechz Tweets

    Pinterest on OJPS

    Help ? Google Reviews 11