A sputum sample was received from a young woman with a past history of pulmonary mycobacterial disease (< 6 months). She has treated 6 month with first line anti tuberculosis drugs based on clinical symptoms and AFB microscopy (AFB positive) and she has cured. Next time, she came with cough and mild fever again. Molecular based laboratory identification of the sputum specimen confirmed that she has Mycobacterium abscessus infection.
After treating with sodium hydroxide (4%) (Sigma), it was centrifuged at 3000 g under refrigerated conditions (at 4°C). The centrifuged deposit was diluted in 1 ml of sterile distilled water to prepare the bacterial suspension. Two slopes of the Lowenstein–Jensen (L-J) medium (Difco), one containing paranitrobenzoic acid (PNB, Sigma) and 7H9 broth medium were inoculated with 100 μl of the bacterial suspension. A small portion of the bacterial suspension (~20 μl) was examined microscopically, using the Ziehl-Neelsen (ZN) stain, to determine the presence of acid fast bacilli (AFB). The inoculated media were incubated at 37°C in a 5% CO2 incubator. The phenotypic characters of colonies were observed and smears were prepared from cultures grown on all 3 media. Culture isolates were tested by nitrate reductase test.
Keywords:
Published on: Dec 31, 2015 Pages: 18-19
Full Text PDF
Full Text HTML
DOI: 10.17352/jcmbt.000004
CrossMark
Publons
Harvard Library HOLLIS
Search IT
Semantic Scholar
Get Citation
Base Search
Scilit
OAI-PMH
ResearchGate
Academic Microsoft
GrowKudos
Universite de Paris
UW Libraries
SJSU King Library
SJSU King Library
NUS Library
McGill
DET KGL BIBLiOTEK
JCU Discovery
Universidad De Lima
WorldCat
VU on WorldCat
PTZ: We're glad you're here. Please click "create a new query" if you are a new visitor to our website and need further information from us.
If you are already a member of our network and need to keep track of any developments regarding a question you have already submitted, click "take me to my Query."