Research progress of near-infrared fl uorescence immunoassay

Xiao Hui Chang, Jie Zhang, Lin Huan Wu, Yan Kun Peng, Xiang Ying Yang, Xiao Lin Li, Ai Jin Ma*, Jun Cai Ma and Guang Quan Chen

Near-infrared fl uorescence immunoassay has been widely studied in the bio analytical fi eld. This

review mainly introduces the basic principles of near-infrared spectroscopy and near-infrared detection

technology, and summarizes the properties, characteristics and recent improvement of optical properties

and signal intensity of three near-infrared fl uorescence probes (i.e. organic fl uorophores, quantum dots

and rare earth compounds). We describe the applications of near-infrared fl uorescence technology in

immunoassay, and prospect the application potential of lateral fl ow assay (LFA) based on this probe in

the rapid detection of pathogens. Our team intends to establish a new platform which has highly sensitive

near-infrared fl uorescence probes (NIFPs) combined with portable and simple immunochromatographic

test strips (ICTSs) for rapid detection of food borne viruses. This will provide technical support for a rapid

detection on the port.

Practical applications

The applications of near-infrared fl uorescence probes (NIFPs) in immunological analysis and clinically

important biomarkers were also elaborated. Moreover, NIFPs-based immunoassay adaptable for rapid

detection of foodborne pathogens was also forecasted. In 2017, our team has developed an approach

for detecting pathogens such as Salmonella, Vibrio parahaemolyticus, Vibrio cholerae and Listeria

monocytogenes by near-infrared immunoassay.

Keywords:

Published on: Feb 27, 2019 Pages: 1-7

Full Text PDF Full Text HTML DOI: 10.17352/2455-3492.000028
CrossMark Publons Harvard Library HOLLIS Search IT Semantic Scholar Get Citation Base Search Scilit OAI-PMH ResearchGate Academic Microsoft GrowKudos Universite de Paris UW Libraries SJSU King Library SJSU King Library NUS Library McGill DET KGL BIBLiOTEK JCU Discovery Universidad De Lima WorldCat VU on WorldCat