Important Factors Influencing Protein Crystallization

Mohnad Abdalla*, Wafa Ali Eltayb, Abdus Samad, TIM Dafaalla

The solution of crystallization problem was introduced around twenty years ago, with the introduction of crystallization screening methods. Here reported some of the factors which affect protein crystallization, solubility, Concentration of precipitant, concentration of macromolecule, ionic strength, pH, temperature, and organism source of macromolecules, reducing or oxidizing environment,additives, ligands, presence of substrates, inhibitors, coenzymes, metal ions and rate of equilibration.The aim of this paper to give very helpful advice for crystallization.

Keywords: Proteins; Crystallization; Isoelectric point; pH; Solubility; Stability

Published on: Sep 30, 2016 Pages: 25-28

Full Text PDF Full Text HTML DOI: 10.17352/gjbbs.000008
CrossMark Publons Harvard Library HOLLIS Search IT Semantic Scholar Get Citation Base Search Scilit OAI-PMH ResearchGate Academic Microsoft GrowKudos Universite de Paris UW Libraries SJSU King Library SJSU King Library NUS Library McGill DET KGL BIBLiOTEK JCU Discovery Universidad De Lima WorldCat VU on WorldCat