Evaluation of Dopamine Receptor Integrity after Sevofl urane Exposure in Neonatal Rat Brain Using Positron Emission Tomography

Qi Yin, Merle G. Paule, Tucker A. Patterson, Fang Liu, Charles M. Fogle, Scott M. Apana, Marc Berridge, William Slikker Jr, Cheng Wang and Xuan Zhang*

Aims: The volatile general anesthetic sevofl urane is commonly used across all ages in the clinic. Sevofl urane-induced neurotoxic effects on the developing dopaminergic system are still unclear. The aim of this study was to evaluate the integrity of the D2/D3 receptor in developing rat brain utilizing molecular imaging techniques. 

Method: Positron Emission Tomography (PET) coupled with Computerized Tomography (CT) approaches were used to evaluate the effects of developmental sevofl urane exposure on D2/D3 receptors in rat pups. The PET radiotracer, [18F]-fallypride, was used to examine whether dopamine receptors were affected by prolonged sevofl urane-exposure during the brain growth spurt. Six postnatal day (PND) 7 rats in the experimental group were exposed to sevofl urane at the clinically-relevant concentration of 2.5% (v/v) for 8 hours, and an equal number of control animals were exposed to room air for 8 hours. MicroPET/CT scans were sequentially collected on PNDs 14, 21, 28, and 35.

Results: No signifi cant differences in the retention of [18F]-fallypride in striatum were detected between sevofl urane-exposed and control animals at any of the scan times. D2/D3 receptors are not affected by prolonged sevofl urane-induced general anesthesia during development.

Conclusions: Sevofl urane-induced neurotoxicity in the developing rodent brain was not associated with apparent derangements in dopamine D2/D3 receptors.

Keywords: Sevofl urane; Dopamine receptor; Positron emission tomography; Fallypride; General anesthetic

Published on: Jan 18, 2018 Pages: 1-6

Full Text PDF Full Text HTML DOI: 10.17352/2455-3476.000040
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