Fabrication and Characterization of HER2 Cell Receptor-Targeted Indocyanine Green-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles

Yu-Hsiang Lee*, Yun-Han Lai

Introduction: The aim of this study is to fabricate and characterize human epidermal growth factor receptor 2 (HER2)-targeted indocyanine green (ICG)-loaded poly (lactic-co-glycolic acid (PLGA) nanoparticles (HIPNPs).

 Methods: The HIPNPs were fabricated by a modified emulsification in association with solvent evaporation approach. The size and surface charge of the manufactured   anoparticles were determined by dynamic light scattering technique. The morphology of the HIPNPs was detected by SEM. The activity of surface anchored anti-HER2 antibodies was detected by spectro fluorometry and fluorescent microscopy. The encapsulation rate of ICG, percentage of ICG content, and the degradation efficiencies of entrapped ICG under different temperatures were measured through UVVis spectrometry.

Results: All HIPNPs exhibited particulate morphology with size of 302 ± 1.8 nm and surface charge of -15 ± 0.15 mV where the polydispersity index was in the range of 0.02 - 0.07. The encapsulation rate of ICG and percentage of ICG content in the HIPNPs were 70% and 23%, respectively. The stability of ICG can be improved after encapsulated into the PLGA nanoparticles, by which the degradation rates of entrapped ICG in 4 o C and 37 o C aqueous medium significantly reduced about 6- and 3-fold, respectively, as compared to the freely dissolved ICG within 48 h. 

Conclusions: We have successfully fabricated and characterized the HIPNPs in this study. Based on the enhanced stability, bioavailability, biocompatibility, and target-ability of the HIPNPs, the developed ICG Nano-carriers exhibited a high potential for use in near infrared-based diagnostics and therapeutics in vitro and/or in vivo for HER2-expressing cells. 

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Published on: Sep 28, 2015 Pages: 15-20

Full Text PDF Full Text HTML DOI: 10.17352/abse.000003
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