1. LC/MS: A Hyphenated Chromatographic Technique

      Kumar Pratyush*, Priya Dule, A. J. Asnani, S. M. Dhole

      Pharmaceutical chemistry is the core branch of pharmacy education and research. It can be categorized as synthesis of new drug molecule, its analysis and pharmacological studies. High performance liquid chromatography is the highly advantageous technique to analyze the sample. Mass spectrometry is method used to quantify the sample. High performance liquid chromatography coupled with mass spectrometry (LC/MS) is a key enabling technology for the detection and characterization of organ- ic molecules, providing the analytical chemist with one of the most powerful analytical tools of modern times. With advancements in ionization methods and instrumentation, liquid chromatography/mass spectrometry (LC/MS) has become a powerful technology for the characterization of macromolecule.

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    2. Docking Studies: A Tool for Computer Aided Drug Design

      Priya Dule*, Kumar Pratyush, D. R. Chaple, A. J. Asnani, S. M. Dhole

      Molecular modeling has become a valuable and essential tool to medicinal chemists in the drug design process. Molecular modeling describes the generation, manipulation or representation of three-dimensional structures, all theoretical method and computational techniques used to model or mimic behaviour of molecule. It involves a range of computerized techniques based on theoretical chemistry methods and experimental data to predict molecular and biological properties. Docking studies are used to find best matching between two molecules. The major aspects of the docking studies are protein flexibility, ligand sampling, and scoring functions.

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    3. Nano-Emulsion: An Emerging Carrier for Drug Delivery

      Rahul Tiwari*, K. Kesavan

      Nano-emulsions, as non-equilibrium systems, present characteristics and properties which depend not only on composition but also on the preparation method. Nano-emulsion droplet sizes fall typically in the range of less than 1000 nm and show narrow size distributions.Nano-emulsions report their formation by dispersion or high-energy emulsification methods, an increased interest is observed in the study of nano-emulsion formation by condensation or low-energy emulsification methods (based on the phase transitions that take place during the emulsification process). Nano-emulsions are proposed for numerous applications in pharmacy as drug delivery systems because of their capacity of solubilizing non polar active compounds.

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    4. Nanostructured Lipid Carriers: A Novel Generation of Solid Lipid Nanoparticles

      Jaiswal R. S.*, Sasankar P. R., Amarwani S. D, Bakhle S.S.

      NanostructuredLipid Carriers (NLC) are the new generation of lipid nanoparticles, attracting major attention as novel colloidal drug carriers. NLC were developed to overcome the limitations associated with the SLN. SLN consist of solid lipids, while NLC consist of a mixture of specially blended solid lipid (long chain) with liquid lipid (short chain), preferably in a ratio of 70:30 up to a ratio of 99.9:0.1. Commonly observed disadvantages of SLN include limited drug-loading capacity, drug expulsion during storage, and relatively high water content in the dispersions (70–99.9%). As compared to SLN, NLC have a higher drug-loading capacity for a number of active compounds, and avoid or minimize potential expulsion of active compounds during storage.

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    5. Nanotheranostics and Pharmacogenomics for the Development of Personalized Medicine

      Sanjay Kumar Bharti* and Debarshi Kar Mahapatra

      Nanotheranostics, the integration of diagnostic and therapeutic function in one system using the versatile strategy of nanotechnology, is extremely attractive for personalized medicine. The discovery of genetic, genomic and clinical biomarkers have revolutionized the treatment option in the form of personalized medicine (PM) which allows us to accurately predict a person’s susceptibility/progression of disease, the patient’s response to therapy, and maximize the therapeutic outcome for a particular patient. It aims to provide right treatment, to the right patient, at the right time, at right cost. "One size does not fit all" is the prime reason for the evolution of PM, which emphasizes on genetic makeup of individuals that can be correlated with difference in drug therapy.

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    6. 66-The-Potential-of-Polymers-on-Intestinal-Permeabiligy-and-PH-Sensitivity-of-Nanoparticles-of-a-Camptothecin-Derivative-An-Ex-vivo-Study.pdf

      Shilpi Prasad*, J.S. Dangi

      SN-38 (7-ethyl-10-hydroxycamptothecin) is a camptothecin derivative is currently being investigated for use in the treatment of metastatic colon cancer. It is potentially cytototoxic but it is insoluble in pharmaceutically acceptable solvents and has low bioavailability. Polymeric nanoparticles can be explored for delivery of SN-38 to colon area for effective treatment of colon cancer. The aim of the present study is to see the effect of biodegradable and non biodegradable polymers on intestinal permeability of drug across colon region and pH sensitivity of nanoparticles. In the present approach chitosan coated PLGA (Poly-lactic-co-glycolicacid) nanoparticles (PCNP) and chitosan coated ES-100 (Eudragit S-100) nanoparticles (ECNP) were prepared by emulsion-solvent evaporation method with modifications.

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    7. First Principles Study of Thermal and Vibrationalfrequencies and Bonding Analysis in Oxides (M2O) Superosides (MO2) and Ozonides (MO3); (M=Li, Na, K)

      Shiba Subedi*, Jeevan Jyoti Nakarmi

      First-principles calculations in order to study the stability of oxides, superoxide and ozonide of alkali metals (Li, Na, K) has been performed by HF cluster procedure implemented by Gaussian 09 sets of programs with the choice of basis set 6-31G*. The correlation effects in the calculations have been accounted by applying Moller-Plesset second order perturbation approximations (MP2). Our calculation shows that when increasing the bond length between atoms of molecules, the binding energy of the corresponding system decreases, i.e. the stability of the studied system is inversely proportional to the bond length between the atoms of the corresponding system.

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    8. 68-Isolation-and-Staining-of-Endophytic-Fungus-with-Special-Reference-to-their-Antibacterial-Activity.pdf

      Sonam Soni*, Yamini Patle, Mukesh Sharma, Kushagra Nagori

      Now days a large number of medicine are also prepared from fungi, plants, bacteria etc. But from all of these, fungi play an important role for formation of useful drugs which is used for curing a number of diseases. Fungi are eukaryotic protists that differ from bacteria and other prokaryotes in many ways. Fungi generally show both type of reproduction. They can reproduce sexually or asexually. The simplest type of fungi is unicellular yeast. Fungi consist of thread like structure called hyphae and the mass of hyphae are called mycelium. Depending upon the morphology, the fungi generally divide into three groups like yeast, yeast like fungi, mould and dimorphic fungi.

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    9. Pharmacophore Modeling, Atom Based 3D-QSAR and Docking Studies of Azetidin-2-Ones Derivatives as Tubulin Inhibitors

      Vijay Kumar Patel*, Harish Rajak

      Azetidin-2-ones have been recognized as effective tubulin polymerization inhibitors that bind to the colchicine site on β-tubulin. Energetic based pharmacophore mapping (hybrid structure and ligand based method) explain how the energy parameter from the Glide XP scoring function are plotted onto pharmacophore sites from the docked fragments so as to rank their implication for binding. Pharmacophore and atom based 3D QSAR modeling (ligand based method) were performed on 71 compounds of azetidin-2-ones derivatives as tubulin-binding agents for antitumor activity. Five-point common pharmacophore hypothesis were selected for alignment of all compounds.

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    10. Effects of Nanoparticles & Nanotechnology on Health

      Madhavi Sahu*, Nitish Kumar Verma, Manisha Korram, Pooja Singh, Achal Mishra

      Pharmaceutical nanotechnology has provided diagnosis and focused treatment of disease at a molecular level and pharmaceutical nanotechnology is most innovative and highly specialized field, which will revolutionize the pharmaceutical industry in near future. Pharmaceutical nanotechnology presents revolutionary opportunities to fight against many diseases. It helps in detecting the antigen associated with diseases such as cancer, diabetes mellitus, neurodegenerative diseases, as well as detecting the microorganisms and viruses associated with infections.

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